广西中医药大学学报2025，Vol.28Issue(6)：7-14,8.
北部湾珊瑚礁区海绵中可培养细菌多样性及生物活性研究
Diversity and Bioactivities of Cultivable Bacteria Isolated from Sponges in the Coral Reef Reserve of the Beibu Gulf
摘要
Abstract
[Objective]To explore thecultivable microbial resources in sponges from the coral reef reserve of the Beibu Gulf and investigate their antibacterial and antitumor activities.[Methods]Fifteen sponge specimens were col-lected from the coral reef reserves of Xieyang Island and the Leizhou Peninsula in the Beibu Gulf.Twelve distinct bacte-rial isolation medium were employed for microbial isolation and cultivation using the dilution plating technique.Bacteri-al diversity was analyzed based on 16S rRNA gene sequencing.After primary antibacterial screening by the agar punch-ing method,the candidates showed antibacterial active were cultivated by fermentation.Then the secondary antibacteri-al screening and anti-prostate cancer active screening were applied on crude extracts from the above fermentation with MTT assay and filtering paper diffusion method.[Results]In total,235 cultivable bacteria representing 33 orders,61 families,and 99 genera were isolated from these 15 sponge specimens.Two species are proposed as putative novel spe-cies.The crude extracts of 21 species exhibited inhibitory activity against 10 pathogenic bacteria.The crude extracts of 8 species showed significant inhibitory activity against prostate cancer cells PC-3 and 22Rv1.[Conclusion]Sponges from the coral reef reserves of the Beibu Gulf contain richdiverse cultivable bacterial resources.Several isolated bacteri-al strains exhibit notable antibacterial and anti-prostate cancer activities,providing valuable strain resources to support the discovery and development of novel antibiotics and anticancer marine drugs.关键词
海绵/珊瑚礁保护区/北部湾/细菌多样性/抑菌活性/抗肿瘤活性Key words
sponge/coral reef reserve/Beibu Gulf/bacterial diversity/antibacterial activity/antitumor activity分类
医药卫生引用本文复制引用
高梦蝶,李蜜,徐淑芬,李柯然,兰建洲,易湘茜,高程海..北部湾珊瑚礁区海绵中可培养细菌多样性及生物活性研究[J].广西中医药大学学报,2025,28(6):7-14,8.基金项目
国家自然科学基金广西区域联合基金项目(编号:U20A20101) （编号:U20A20101）
广西重点研发计划项目(编号:桂科AB24010109) （编号:桂科AB24010109）
广西自然科学基金青年基金项目(编号:2024GXNSFBA010366) （编号:2024GXNSFBA010366）
广西中医药大学"桂派中医药传承创新团队"项目(编号:2022A007) （编号:2022A007）
广西中医药大学桂派杏林拔尖人才资助项目(编号:2022C008) （编号:2022C008）
广西中医药大学岐黄工程高层次人才团队培育项目(编号:202404) （编号:202404）
