广西中医药大学学报2025，Vol.28Issue(6)：28-32,5.
胄甲海绵共附生放线菌Streptomyces albidoflavus LHW3101次级代谢产物及其抑菌活性研究
Study on the Secondary Metabolites of the Dactylospongia elegans-Associated Actinomycete Streptomyces albidoflavus LHW3101 and their Antibacterial Activities
摘要
Abstract
[Objective]To study the secondary metabolitesof actinomycete Streptomyces albidoflavus LHW3101 as-sociated with Dactylospongia elegans and their antibacterial activities.[Methods]The compounds in the fermentation products of Streptomyces albidoflavus LHW3101 were isolated and purified by gel column chromatography,high-perfor-mance liquid chromatography,and other techniques.The structures of these compounds were determined through NMR,HRMS/MS,Global Natural Products Social Molecular Networking(GNPS)database,and literature comparison.The an-tibacterial activities of the compounds were evaluated by the broth microdilution method.[Results]A total of 10 mono-meric compounds were isolated and identified,namely Surugamide B～E(1～4),cyclo-(L-Val-L-Ile)(5),cyclo-(L-Val-L-Leu)(6),cyclo-(L-Ile-L-Met)(7),cyclo-(L-Leu-L-Met)(8),cyclo-(L-Trp-D-Val)(9),and N-phenethyl-acetamide(10).The minimum inhibitory concentrations(MIC)of compounds 1～4 against Staphylococcus aureus were 16.0 μg/ml,32.0 μg/ml,32.0 μg/ml,and 64.0 μg/ml,respectively.[Conclusion]The secondary metabolites of Streptomyces albidoflavus LHW3101 associated with Dactylospongia elegans contain various cyclic peptide compounds,and Compound 1～4 exhibit moderate inhibitory activity against Staphylococcus aureus.关键词
海绵放线菌/次级代谢产物/Surugamides/GNPS/环肽/金黄色葡萄球菌Key words
sponge-associated actinomycete/secondary metabolites/Surugamides/GNPS/cyclic peptide/Staphy-lococcus aureus分类
医药卫生引用本文复制引用
陈龙,林霄,薛培英,王雅楠,杨炜婵,杜永雪,朱婧琦,罗小卫,高程海,刘永宏..胄甲海绵共附生放线菌Streptomyces albidoflavus LHW3101次级代谢产物及其抑菌活性研究[J].广西中医药大学学报,2025,28(6):28-32,5.基金项目
国家自然科学基金项目(编号:82560684、82160669、U24A20808) （编号:82560684、82160669、U24A20808）
