海南医科大学学报2025，Vol.31Issue(21)：1621-1629,9.DOI:10.13210/j.cnki.jhmu.20250327.006
基于miR-155/FGF7通路介导的巨噬细胞极化探讨顾步汤改善糖尿病足溃疡的分子机制
Exploration of the molecular mechanism of Gubu Decoction in regulating miR-155/FGF7 pathway mediated macrophage polarization to treat diabetic foot ulcers
摘要
Abstract
Objective:To explore the mechanism of Gubu Decoction(GBD)in improving diabetes foot ulcer(DFU)by regu-lating macrophage polarization through the miR-155/FGF7 signal pathway.Methods:SD rat diabetes model was established by in-traperitoneal injection of 55 mg/kg streptozotocin,and the wound model of difficult DFU was established.The experiment was di-vided into 5 groups:the blank group,the model group,the GBD group,the miR-155 inhibitor group,and the GBD+miR-155 in-hibitor group.The GBD group was given GBD concentrate(90 mL/kg)by gavage,while the blank group and model group were given distilled water(90 mL/kg)by gavage.The miR-155 inhibitor group was injected intraperitoneally with miR-155 inhibitor(20 μmol/L),while the GBD+miR-155 inhibitor group was injected intraperitoneally and gavage simultaneously.The pathologi-cal morphology of ulcer tissues in each group of rats was observed by H&E staining.The levels of inflammatory factors(IL-6,IL-1β,IL-10,IL-4)in the serum of each group were detected by enzyme-linked immunosorbent assay.Flow cytometry was used to analyze the expression of CD206 and macrophage polarization marker molecules in ulcer tissues.qPCR and Western blot were used to detect the expression of miR-155,iNOS,COX-2,Arg-1,YM-1,and FGF7.Results:Compared to the blank group,the levels of IL-6,IL-1β,CD68,iNOS,COX-2,miR-155 expression,and CD68/CD206 ratio were significantly increased in the model group.The levels of IL-4,IL-10,CD206,Arg-1,YM-1,and FGF7 were significantly reduced(P<0.01).Compared to the model group,the levels of IL-6,IL-1β,CD68,iNOS,COX-2,miR-155 expression,and CD68/CD206 ratio were signifi-cantly decreased in the GBD group,while the levels of IL-4,IL-10,CD206,Arg-1,YM-1,and FGF7 expression were signifi-cantly increased(P<0.05).Conclusion:GBD upregulates FGF7 expression by targeting miR-155,promotes M2 polarization of macrophages,inhibits inflammation,and ultimately improves DFU symptoms.关键词
顾步汤/糖尿病足溃疡/巨噬细胞极化/miR155/FGF7通路Key words
Gubu Decoction/Diabetic foot ulcers/Macrophage polarization/miR-155/FGF-7 pathway分类
医药卫生引用本文复制引用
王继雪,杨稀瑞,周涛,燕树勋,王思琦,夏联恒,袁星星..基于miR-155/FGF7通路介导的巨噬细胞极化探讨顾步汤改善糖尿病足溃疡的分子机制[J].海南医科大学学报,2025,31(21):1621-1629,9.基金项目
This study was supported by the National Natural Science Foundation of China(82205195,82205117) （82205195,82205117）
Henan Province Postdoctoral Research Project(HN2024080,HN2024083) （HN2024080,HN2024083）
Henan Province Medical Science and Technology Key Project(LHGJ2024067,LHGJ20230686) （LHGJ2024067,LHGJ20230686）
Research Special Project of Henan Province Clinical Research Base of Traditional Chinese Medicine(2022JDZX127,2022JDZX135) 国家自然科学基金资助项目(82205195,82205117) （2022JDZX127,2022JDZX135）
河南省博士后科研项目(HN2024080,HN2024083) （HN2024080,HN2024083）
河南省医学科技攻关计划项目(LHGJ2024067,LHGJ20230686) （LHGJ2024067,LHGJ20230686）
河南省中医临床研究基地科研专项(2022JDZX127,2022JDZX135) （2022JDZX127,2022JDZX135）
