海南医科大学学报2025，Vol.31Issue(21)：1661-1671,11.DOI:10.13210/j.cnki.jhmu.20241206.001
基于转录组测序探讨光动力疗法对人牙周炎性组织mRNA表达谱的影响
Exploring the effect of photodynamic therapy on mRNA expression profile of human periodontic tissue based on RNA sequencing
摘要
Abstract
Objective:To explore the effect of photodynamic therapy(PDT)on gene expression in human periodontitis tissues based on RNA sequencing,analyze key differentially expressed genes(DEGs),and explore the potential mechanism of PDT.Methods:A total of 5 cases of healthy periodontal tissue,5 cases of periodontic tissue,and 5 cases of periodontic tissue treated with PDT were collected clinically,and RNA sequencing was performed.Bioinformatics analysis was used for hub gene screening and enrichment analysis.Another 15 cases of tissues for each group were collected and validated the sequencing results using RT-qPCR.Results:A total of 6 526 DEGs were detected between healthy periodontal tissue and periodontic tissue,and 1 209 DEGs were detected between periodontic tissue and periodontic tissue after PDT treatment,and 72 immune related differentially expressed genes were screened.GO enrichment analysis included biological processes such as leukocyte migration and bacterial de-fense response;KEGG pathway analysis mainly involved cytokine-cytokine receptor interactions,JAK-STAT signaling pathway,IL-17 signaling pathway,and other pathways.A PPI network was constructed and screen out 6 hub genes(S100A7,LCN2,LTF,DEFB4A,S100A12,S100A8).The RT-qPCR validation results were consistent with the sequencing results.Conclu-sion:The study determines the expression profile of mRNA in periodontitis tissue after PDT treatment,and identifies of relevant hub genes and functional analysis,can provide new ideas for studying the potential mechanism of PDT regulating of periodontitis tissue.关键词
牙周炎/光动力疗法/转录组测序/牙周炎性组织Key words
Periodontitis/Photodynamic therapy/RNA sequencing/Periodontic tissue分类
口腔医学引用本文复制引用
马潇,傅亚婷,黄佳惠,孙媛媛,刘双环,刘华,昆得孜·杜肯..基于转录组测序探讨光动力疗法对人牙周炎性组织mRNA表达谱的影响[J].海南医科大学学报,2025,31(21):1661-1671,11.基金项目
This study was supported by the Research Project of Xinjiang Second Medical College(ZR202406) （ZR202406）
Urumqi Traditional Chinese Medicine Technology Innovation Project(ZYYMS-10) （ZYYMS-10）
Urumqi Municipal Health Commission Science and Technology Plan Project(202322) 新疆第二医学院科研项目(ZR202406) （202322）
乌鲁木齐市中医药科技创新项目(ZYYMS-10) （ZYYMS-10）
乌鲁木齐市卫生健康委科技计划项目(202322) （202322）
