湖南中医药大学学报2025，Vol.45Issue(11)：2054-2062,9.DOI:10.3969/j.issn.1674-070X.2025.11.005
青附蠲痹汤治疗类风湿关节炎病证作用的网络药理学分析与实验验证
Network pharmacology and experimental study on Qingfu Juanbi Decoction in treating rheumatoid arthritis of wind-cold-damp pattern
摘要
Abstract
Objective To identify the active ingredients,potential targets,and key pathways of Qingfu Juanbi Decoction(QFJBD)in treating rheumatoid arthritis(RA)of wind-cold-damp Bi-impediment pattern based on network pharmacology,and to validate its pharmacological effects through animal experiments.Methods(1)Network pharmacology research:Active ingredients in QFJBD that comply with Lipinski's Rule of Five and exhibit good oral bioavailability were screened using SwissADME.Potential targets of these ingredients were predicted via Swiss Target Prediction and Batman-TCM databases.A"herb-compound-target"network was constructed using Cytoscape 3.10.0.A protein-protein interaction(PPI)network was established via the STRING database,and core targets were identified using CytoHubba plugin according to three centrality metrics(Degree,Betweenness,and Closeness).GO functional and KEGG pathway enrichment analyses were performed with the ClusterProfiler package.(2)Animal experimental validation:An adjuvant-induced arthritis(AIA)rat model of wind-cold-damp Bi-impediment pattern was established.Model rats were randomly assigned into the model group,methotrexate(MTX)group(1.0 mg/kg,three times per week),and low-,medium-,and high-dose QFJBD groups[4.59,9.18,and 18.36 g/(kg·d),respectively],with six rats in each group.A normal group(n=6)was also included.Medication intervention began on the day after modeling completion and continued until day 42.Paw swelling volume and arthritis scores were measured every three days.Serum levels of IL-1β,IL-6,IL-10,TNF-α,and IL-17A were detected by ELISA after the intervention.Histopathological changes in joint tissues were observed using HE,Masson,and SO-FG staining.Results Network pharmacology screening identified 24 active compounds and 331 potential therapeutic targets in QFJBD.KEGG enrichment analysis revealed that signaling pathways such as PI3K-Akt and mTOR may play pivotal roles.Animal experiments demonstrated that,compared with the model group,the high-dose QFJBD group exhibited significantly alleviated paw swelling(P＜0.01)and reduced arthritis scores(P＜0.001).Additionally,the serum levels of IL-1β,IL-6,IL-10,TNF-α,and IL-17A markedly decreased(P＜0.000 1).Histopathological examination indicated that QFJBD significantly inhibited synovial hyperplasia,inflammatory cell infiltration,collagen deposition,and bone/cartilage destruction,showing dose-dependent amelioration.Conclusion QFJBD exerts significant therapeutic effects on RA of wind-cold-damp pattern,likely through a synergistic mechanism of action involving multiple components,multiple targets,and multiple pathways.关键词
类风湿关节炎/青附蠲痹汤/风寒湿痹证/网络药理学/炎症因子Key words
rheumatoid arthritis/Qingfu Juanbi Decoction/wind-cold-damp Bi-impediment pattern/network pharmacolo-gy/inflammatory factor分类
医药卫生引用本文复制引用
戴宗顺,唐媛媛,尧忠柳,彭清华,林也,蔡雄..青附蠲痹汤治疗类风湿关节炎病证作用的网络药理学分析与实验验证[J].湖南中医药大学学报,2025,45(11):2054-2062,9.基金项目
国家自然科学基金面上项目(82274506) （82274506）
湖南省自然科学基金项目(2025JJ90123) （2025JJ90123）
长沙市自然科学基金项目(kq2208499) （kq2208499）
湖南中医药大学院校联合课题重点项目(2022XYLH158). （2022XYLH158）
