湖南中医药大学学报2025，Vol.45Issue(11)：2090-2097,8.DOI:10.3969/j.issn.1674-070X.2025.11.009
三七皂苷R1通过激活AMPK/mTOR/ULK1信号通路促进脂多糖诱导的牙周炎细胞模型自噬
Panax notoginseng saponin R1 activates the AMPK/mTOR/ULK1 signaling pathway to promote autophagy in a lipopolysaccharide-induced periodontitis cell model
摘要
Abstract
Objective To investigate the effects of Panax notoginseng saponin R1(NGR1)on autophagy in lipopolysaccharide(LPS)-induced periodontitis cells via the adenosine 5'-monophosphate-activated protein kinase(AMPK)/mammalian rapamycin target protein(mTOR)/Unc-51 like kinase 1(ULK1)signaling pathway.Methods Human periodontal ligament fibroblasts(hPDLFs)were treated with 100 ng/mL LPS for 24 h to establish a periodontitis cell model.The periodontitis cells were divided into control group,LPS group,LPS+NGR1 group,and LPS+NGR1+AMPK inhibitor Compound C group.Flow cytometry was used to check apoptosis of the cells.The protein expression levels of Bcl-2,Bax,cleaved Caspase-3,Beclin-1,p62,p-AMPK,p-mTOR,and p-ULK1,as well as the LC3-Ⅱ/Ⅰ ratio,were determined by Western blot.Immunofluorescence staining assay was used to examine the protein expression level of LC3.Results Compared with the control group,the LPS group exhibited a significant increase in the apoptosis rate of cells(P＜0.05).The protein expression levels of Bcl-2,Beclin-1,p-AMPK and p-ULK1 in cells,as well as the LC3-Ⅱ/Ⅰ ratio in cells decreased(P＜0.05).Conversely,the protein expression levels of Bax,cleaved Caspase-3,p62,and p-mTOR in cells were elevated(P＜0.05).Compared with the LPS group,the apoptosis rate of cells in LPS+Panax notoginseng saponin R1 group and LPS+Panax notoginseng saponin R1+Compound C group decreased(P＜0.05).The protein expression levels of Bcl-2,Beclin-1,p-AMPK,and p-ULK1,as well as the LC3-Ⅱ/Ⅰ ratio,significantly increased(P＜0.05).Conversely,the protein expression levels of Bax,cleaved Caspase-3,p62,and p-mTOR significantly decreased(P＜0.05).Compared with the LPS+notoginsenoside R1 group,the cells in the LPS+notoginsenoside R1+Compound C group exhibited an increased apoptosis rate(P＜0.05),along with decreased levels of Bcl-2,Beclin-1,the ratio of LC3-Ⅱ/Ⅰ,p-AMPK,and p-ULK1(P＜0.05),while showing increased levels of Bax,cleaved Caspase-3,p62,and p-mTOR(P＜0.05).Conclusion Panax notoginseng saponin R1 promotes autophagy and inhibits apoptosis of LPS-induced periodontitis cells,and its mechanism may be related to the activation of AMPK/mTOR/ULK1 signaling pathway.关键词
牙周炎/人牙周膜成纤维细胞/三七皂苷R1/AMPK/mTOR/ULK1信号通路/自噬/凋亡Key words
periodontitis/human periodontal ligament fibroblasts/Panax notoginseng saponin R1/AMPK/mTOR/ULK1 sig-naling pathway/autophagy/apoptosis分类
医药卫生引用本文复制引用
顾龙龙,单妍,梁璇,李荣,谢薇..三七皂苷R1通过激活AMPK/mTOR/ULK1信号通路促进脂多糖诱导的牙周炎细胞模型自噬[J].湖南中医药大学学报,2025,45(11):2090-2097,8.基金项目
昆明医科大学海源学院2024年科学研究基金项目(2024HY013). （2024HY013）
