湖南中医药大学学报2025，Vol.45Issue(11)：2116-2124,9.DOI:10.3969/j.issn.1674-070X.2025.11.012
针刺对动脉粥样硬化兔脂质代谢及肠道菌群的影响
Influence of acupuncture on lipid metabolism and gut microbiota in rabbits with atherosclerosis
摘要
Abstract
Objective To explore the effects of acupuncture on lipid metabolism and gut microbiota in rabbits with atherosclerosis(AS)induced by a high-fat diet and its mechanism of action.Methods Twenty-four New Zealand rabbits were randomly divided into blank group,model group,and acupuncture group,with 8 rabbits in each group.The blank group was fed with a normal diet,the model group was fed with a high-fat diet,and the acupuncture group was fed with high-fat diet combined with daily acupuncture intervention,once daily for 12 weeks.Serum levels of total cholesterol(TC),triglycerides(TG),low-density lipoprotein cholesterol(LDL-C),and high-density lipoprotein cholesterol(HDL-C)were measured using a fully automated biochemistry analyzer.Pathological changes in the rabbit aortas and plaque areas were observed through hematoxylin-eosin(HE)staining and oil red O staining.The structure and function of the gut microbiota were analyzed by 16S rDNA sequencing.Results Compared with the blank group,the levels of TC,TG,and LDL-C increased(P＜0.01),the HDL-C levels decreased(P＜0.01),and the ratio of thoracic aortic plaque area increased(P＜0.05)in the model group.Compared with the model group,the levels of TC,TG,and LDL-C decreased(P＜0.01),the HDL-C levels increased(P＜0.05),and the ratio of thoracic aortic plaque area decreased(P＜0.05)in the acupuncture group.The results of 16S rDNA sequencing indicated that acupuncture could modulate the gut microbiota structure and influence both α and β diversity in AS rabbits.Compared with the blank group,the model group demonstrated increased relative abundances of Firmicutes and the NK4A214_group genus(P＜0.05),while showing decreased relative abundances of Bacteroidota,Cyanobacteria,Muribaculacea,and Bacteroides(P＜0.05).In comparison with the model group,the acupuncture group exhibited increased relative abundances of Bacteroidota,Cyanobacteria,Muribaculacea,and Bacteroides(P＜0.05),along with a decreased relative abundance of the NK4A214_group genus(P＜0.05).KEGG functional predictions revealed that,compared with the blank group,the model group had reduced relative abundances of Biosynthesis of other secondary metabolites and Glycan biosynthesis and metabolism(P＜0.05).Conversely,compared with the model group,the acupuncture group showed an increased relative abundance of Glycan biosynthesis and metabolism(P＜0.05).Conclusion Acupuncture may improve lipid metabolism and alleviate aortic injury in AS rabbits by modulating the diversity and structure of the gut microbiota.Meanwhile,it may promote endothelial function recovery by influencing carbohydrate chain biosynthesis and metabolic functions,thereby exerting anti-atherosclerotic effects.关键词
动脉粥样硬化/针刺/16S rDNA测序/肠道菌群/脂质代谢Key words
atherosclerosis/acupuncture/16S rDNA sequencing/gut microbiota/lipid metabolism分类
中医学引用本文复制引用
肖孟霞,刘超,田安哲,郭城,刘红华,陈昕羽,易洪芬,葛君芸,刘迈兰..针刺对动脉粥样硬化兔脂质代谢及肠道菌群的影响[J].湖南中医药大学学报,2025,45(11):2116-2124,9.基金项目
国家自然科学基金项目(82074559) （82074559）
湖南省重点研发计划项目(2024JK2130) （2024JK2130）
湖南省青年科技创新人才项目(2022RC1222) （2022RC1222）
长沙市杰出创新青年培养计划项目(kq1905036) （kq1905036）
湖南省教育厅重点研发项目(24A0282). （24A0282）
