湖南农业大学学报（自然科学版）2025，Vol.51Issue(5)：1-9,35,10.DOI:10.13331/j.cnki.jhau.2025.05.001
玉米铁氧还蛋白ZmFdx5基因的克隆及其在逆境胁迫下的表达分析
Cloning and expression analysis of the maize ferredoxin gene ZmFdx5 under stress conditions
摘要
Abstract
To investigate the structural characteristics of the ferredoxin-encoding gene ZmFdx5 in tropical maize(Zea mays L.)and elucidate its functional role in abiotic stress responses,the ZmFdx5 gene was successfully cloned from the tropical maize inbred line CML288.Comprehensive bioinformatics analysis and phylogenetic reconstruction were performed to characterize the ZmFdx5 protein,while its expression patterns under drought and salt stress conditions were systematically examined.The results showed that the ZmFdx5 gene comprised a 585 bp open reading frame encoding a polypeptide of 194 amino acids.The deduced ZmFdx5 protein contained a conserved fer2 superfamily domain and was characterized as a hydrophilic,unstable protein that lacks both a signal peptide and transmembrane domains.The protein was predominantly composed of random coils and α-helices.The ZmFdx5 protein may functionally interact with proteins including NAR1 and ferredoxin-thioredoxin reductase(FTRC).ZmFdx5 was located within the photosynthetic ferredoxin group,further supporting its classification as a photosynthetic-type ferredoxin in maize.Furthermore,ZmFdx5 exhibited a closer phylogenetic relationship with C4 monocot species such as sorghum and switchgrass.ZmFdx5 was predominantly expressed in leaf tissues and demonstrated significant responsiveness to both drought and salinity stress conditions.关键词
玉米/ZmFdx5/基因克隆/表达模式/逆境胁迫Key words
maize/ZmFdx5/gene cloning/expression pattern/adversity stress分类
农业科学引用本文复制引用
赵高鹏,洪双双,范怡雯,刘泓畅,张泰铭,刘青青,张严玲,王翠玲..玉米铁氧还蛋白ZmFdx5基因的克隆及其在逆境胁迫下的表达分析[J].湖南农业大学学报（自然科学版）,2025,51(5):1-9,35,10.基金项目
国家自然科学基金河南省联合基金项目(U2004153) （U2004153）
河南省科技攻关项目(232102111096) （232102111096）
河南省高等学校重点科研项目(23A210025) （23A210025）
