湖南农业大学学报（自然科学版）2025，Vol.51Issue(5)：10-18,9.DOI:10.13331/j.cnki.jhau.2025.05.002
千穗谷GRF转录因子家族的全基因组鉴定及其对赤霉素的响应模式分析
Genome-wide identification and analysis of the GRF transcription factor family in Amaranthus hypochondriacus and its response to gibberellin
摘要
Abstract
To clarify the role of the AhGRF gene in the growth and development of Amaranthus hypochondriacus,we identified the members of its gene family utilizing the latest genomic data,followed by a comprehensive analysis of their sequences and response patterns to exogenous gibberellin.The results showed that nine genes including AhGRF1-AhGRF9 were identified and they were unevenly distributed on eight chromosomes and can be divided into five subfamilies,with different AhGRF genes exhibiting distinct expression patterns and the vast majority being highly expressed in floral organs.The promoter regions of AhGRFs contained response elements for hormones such as salicylic acid,abscisic acid,methyl jasmonate,gibberellin,and auxin,as well as stress-related elements like those induced by drought and low temperature;promoters of AhGRF2,AhGRF5,AhGRF6,AhGRF8 also carried functional elements associated with endosperm expression,meristem development and palisade mesophyll cell differentiation.Furthermore,gibberellin treatment significantly promoted leaf and stem growth.Except for AhGRF8,which was not expressed in leaves and stems,the expressions of AhGRF7 and AhGRF9 in leaves were suppressed,while the expressions of AhGRF1-AhGRF6 were upregulated at different time points.In stems,relative expressions of all genes were upregulated at 1 hour after gibberellin treatment,displaying a rhythmic expression pattern in multiple genes,which differed from their expression profile in leaves.关键词
千穗谷/生长调节因子(GRF)/基因家族/表达分析/赤霉素Key words
Amaranthus hypochondriacus/growth regulating factor/gene family/expression analysis/gibberellins分类
生物学引用本文复制引用
杨晟,甘锦鑫,周涛,刘宁芳,卢蕊,胡龙兴,徐倩..千穗谷GRF转录因子家族的全基因组鉴定及其对赤霉素的响应模式分析[J].湖南农业大学学报（自然科学版）,2025,51(5):10-18,9.基金项目
国家自然科学基金项目(32001408) （32001408）
湖南省自然科学基金项目(2024JJ5181) （2024JJ5181）
