湖南农业大学学报（自然科学版）2025，Vol.51Issue(5)：36-44,9.DOI:10.13331/j.cnki.jhau.2025.05.005
MATH结构域基因TC1A过表达对植物耐旱性的影响
The effect of overexpression of TC1A gene with MATH domains on plant drought tolerance
摘要
Abstract
Using wild-type Arabidopsis thaliana(Col-0),TC1A-GUS,TC1A-GFP reporter lines,and two independent overexpression lines(OE-TC1A#1 and OE-TC1A#2)as experimental materials,we first characterized the expression pattern and subcellular localization of TC1A—a previously uncharacterized MATH domain gene in Arabidopsis.We then performed integrated phenotypic,physiological,transcriptomic,and hormonal analyses on wild-type and TC1A-overexpressing plants to elucidate the role of TC1A in mediating drought tolerance.Subcellular localization revealed that the TC1A protein localizes to the plasma membrane and nucleus.Transcriptomic analysis indicated that genes differentially expressed between TC1A-overexpressed plants and wild-type(Col-0)plants were predominantly enriched in stress response and hormone signaling pathways.The transcriptional level of TC1A was up-regulated under drought stress,and overexpression of the TC1A gene significantly improved the drought tolerance of Arabidopsis plants.Hormonal profiling revealed no significant difference in ABA content between overexpressed plants and wild-type plants.However,the level of intermediate product 12-oxo-phytodienoic acid(OPDA)in the jasmonic acid synthesis process was significantly increased in TC1A overexpression plants,suggesting that TC1A regulates drought tolerance via OPDA signaling rather than ABA signaling.关键词
拟南芥/MATH结构域/12-氧-植物二烯酸(OPDA)/耐旱性/TC1A基因Key words
Arabidopsis thaliana/MATH domain/12-oxo-phytodienoic acid(OPDA)/drought resistance/TC1A gene分类
生物学引用本文复制引用
石涵,朱文君,肖牧,阮颖,刘春林..MATH结构域基因TC1A过表达对植物耐旱性的影响[J].湖南农业大学学报（自然科学版）,2025,51(5):36-44,9.基金项目
国家自然科学基金委联合重点项目(U20A2028) （U20A2028）
湖南省科技人才托举项目(2022TJ-Y04) （2022TJ-Y04）
