海洋科学2025，Vol.49Issue(4)：25-38,14.DOI:10.11759/hykx20241209001
纳米二氧化钛对海洋硅藻有机磷酸酯累积、转化及毒性的影响
Effects of titanium dioxide nanoparticles on the accumulation,transformation,and toxicity of organophosphate esters in marine diatoms
摘要
Abstract
Metal-based nanoparticles(MNPs)are extensively employed in modern industry and everyday life,rais-ing substantial concerns about their potential risks to ecosystems.However,research on how MNPs influence the biotransformation of coexisting pollutants remains limited.This study explores the effects of nano-titanium dioxide(nTiO₂)on the accumulation,transformation,and toxicity of organophosphate esters(OPEs)in the marine diatom Phaeodactylum tricornutum.Through acute toxicity assays,single-exposure experiments,and co-exposure experi-ments with nTiO₂,it was observed that triphenyl phosphate(TPHP)exhibited significantly greater toxicity than other tested OPEs.In contrast,its primary metabolite,diphenyl phosphate(DPHP),showed markedly reduced tox-icity,indicating a detoxification effect resulting from OPE biotransformation.However,the presence of nTiO₂ sig-nificantly increased the intracellular accumulation of TPHP while reducing DPHP levels,suggesting that nTiO₂ in-hibits the conversion of TPHP to DPHP.This inhibition leads to greater TPHP retention and elevated toxicity in algae.The increased toxicity was reflected in elevated oxidative stress,as evidenced by a significant rise in super-oxide dismutase(SOD)activity and malondialdehyde(MDA)content.Further mechanistic investigations revealed that nTiO₂ enhances the adsorption and enrichment of OPEs on the algal surface while inhibiting CYP450 gene ex-pression,thereby impairing OPE metabolism and intensifying their toxic effects.This study elucidates the inhibito-ry role of nTiO₂ in the biotransformation of OPEs and its contribution to heightened toxicity.It offers new theoreti-cal insights into the interactions between nanoparticles and organic pollutants in marine environments and provides critical data for environmental risk assessment.关键词
三角褐指藻/nTiO2/OPEs/生物转化/机制Key words
Phaeodactylum tricornutum/titanium dioxide nanoparticles/organophosphate esters/biotransformation/mechanism分类
环境科学引用本文复制引用
戴雪田,刘冬妮,杨芷,李建磊,钱伟,朱小山..纳米二氧化钛对海洋硅藻有机磷酸酯累积、转化及毒性的影响[J].海洋科学,2025,49(4):25-38,14.基金项目
国家自然科学基金(42407382) （42407382）
海南省自然科学基金面上项目(323MS011) （323MS011）
海南大学生态文明协同创新中心项目(XTCX2022STC20) （XTCX2022STC20）
海南省科技人才创新项目(KJRC2023C11) The National Natural Science Foundation of China,No.42407382 （KJRC2023C11）
the Hainan Provincial Natural Science Foundation of China,No.323MS011 （）
Hainan University Collaborative Innovation Center for Eco-logical Civilization Project,No.XTCX2022STC20 （）
the Innovational Fund for Scientific and Technological Personnel of Hainan Province,No.KJRC2023C11 （）
