井冈山大学学报（自然科学版）2025，Vol.46Issue(6)：24-31,8.DOI:10.3969/j.issn.1674-8085.2025.06.004
基于金属有机框架材料结合LC-MS/MS-多肽组学的地龙多肽类成分分析
Analysis of pheretima peptide components based on metal-organic frameworks combined with LC-MS/MS and peptidomics
摘要
Abstract
A metal-organic framework material combined with liquid chromatography tandem mass spectrometry(LC-MS/MS)-peptideomics technique was established to analyze the peptide components in Pheretima and its preparations.The metal-organic framework material NU-1000 was used to enrich the peptide components in Pheretima and its preparations.The enriched peptide components were separated and analyzed by LC-MS/MS.The structure of the peptides was identified by De novo software,and their characteristic were compared with the Pheretima database.The activity of the peptides was predicted by using peptide activity prediction software.The results showed that 425 peptides were successfully identified in Pheretima by NU-1000 combined with LC-MS/MS.Among them,141 amino acid sequences of peptides derived from 20 precursor proteins,four peptides FAGDDARP,FAGDDARP,FPSLVGR and FAGDDAARP were matched to five proteins and all of them were actin,which had antithrombotic pharmacological effects.A total of 15 new peptides with potential biological activity were obtained from the peptide earthworm protein tablet.The analysis method of the peptide components in Pheretima and their preparations established in this study is easy to operate and can quickly analyze the peptide components,which is expected to provide the support for the mining and developing the active components of Pheretima peptides.关键词
金属有机框架材料/LC-MS/MS/多肽组学/地龙/多肽Key words
metal-organic framework/LC-MS/MS/peptideomics/Pheretima/peptide分类
化学引用本文复制引用
刘维清,颜松益,胡诗筠,李琦,余丽双..基于金属有机框架材料结合LC-MS/MS-多肽组学的地龙多肽类成分分析[J].井冈山大学学报（自然科学版）,2025,46(6):24-31,8.基金项目
国家自然科学基金项目(82074005) （82074005）
福建省自然科学基金面上项目(2024J01130) （2024J01130）
福建省科技厅科技创新联合资金项目(2024Y9510) （2024Y9510）
