井冈山大学学报（自然科学版）2025，Vol.46Issue(6)：32-55,24.DOI:10.3969/j.issn.1674-8085.2025.06.005
乳酸菌对抗生素和消毒剂的耐受性及相关耐药基因的探究
Tolerance of lactic acid bacteria to antibiotics and disinfectants and the exploration of related resistance genes
摘要
Abstract
Both the broad-spectrum bactericidal properties of disinfectants and the inhibitory effects of antibiotics pose potential threats to the activity,functionality,and probiotic properties of Lactid acid bacteria(LAB).Therefore,it is essential to systematically evaluate the tolerance of lactic acid bacteria to common disinfectants and antibiotics.This study assessed 60 LAB strains isolated from the fermented foods for their tolerance to seven disinfectants and nine antibiotics.The results indicated that,compared to pathogenic bacteria,most LAB exhibited stronger tolerance to disinfectants.Sub-inhibitory concentrations of disinfectants inhibited biofilm formation in most LAB,while sub-inhibitory concentrations of glutaraldehyde tended to promote biofilm formation.This suggests that commonly used disinfectants can eliminate pathogenic bacteria without killing LAB,providing guidance for disinfectant use.LAB were highly sensitive to ampicillin and chloramphenicol,while showing some tolerance to norfloxacin,polymyxin,and sulfamethoxazole.This indicates LAB have a notable antibiotic resistance profile,which includes both natural resistance characteristics and instances of acquired resistance,along with multiple drug resistance and cross-resistance between antibiotics and disinfectants.This study provides an important guidance for disinfection practices in industrial utilizing LAB,such as food and biomedicine,and offers a reference for the use of antibiotics.关键词
乳酸菌/消毒剂/抗生素/耐药性Key words
lactid acid bacteria/disinfectants/antibiotics/resistance分类
生物学引用本文复制引用
袁露,刘蕾,杨耀,张成毅,宋云龙,黎小丽,龙海琪,朱香亭,张景媛,张石磊..乳酸菌对抗生素和消毒剂的耐受性及相关耐药基因的探究[J].井冈山大学学报（自然科学版）,2025,46(6):32-55,24.基金项目
This work was supported by the National Science Foundation of China(32001663) （32001663）
