寄生虫与医学昆虫学报2025，Vol.32Issue(3)：153-159,178,8.DOI:10.3969/j.issn.1005-0507.2025.03.004
白纹伊蚊感染DENV2免疫相关基因的筛选与分析
SCREENING AND EXPRESSION ANALYSIS OF GENES ASSOCIATED WITH INNATE IMMUNITY IN RESPONSE TO DENV2 INFECTION IN AEDES ALBOPICTUS
摘要
Abstract
Objective Aedes albopictus is the primary vector of the dengue virus.Screening and the analysis of immune-related genes in DENV2-infected Ae.albopictus provides a scientific basis for further research on blocking the extrinsic incubation period of the dengue virus.Methods Through the approach of literature mining,thirty-three potential immune-related genes were screened from species such as Aedes aegypti and Anopheles gambiae,which have a close genetic relationship with Ae.albopictus.The protein—protein interaction(PPI)analysis is employed to explore the interaction relationship of the proteins encoded by genes.The alterations in mRNA expression levels of the relevant genes in DENV2-infected Ae.albopictus midgut were detected using the qRT-PCR method.Results The PPI results indicates that TLR and Spz of Ae.albopictus;Rel1,Rel2,DefC,Spz,PIWI,Ago2,DOME and HOP of Ae.aegypti;and Rel1,Rel2,CACT,STAT and DOME of An.gambiae exhibit PPI relationships.After Ae.albopictus was infected with DENV2,10 genes showed significant difference in expression.Ago3(7.39,P<0.05),DOMEa(1.63,P<0.01),DOMEb(21.29,P<0.001),and TLRb(1.61,P<0.05)were significantly up-regulated.Rel1(0.62,P<0.001),CACTl(0.65,P<0.001),Rel2a(0.65,P<0.01),Rel2b(0.24,P<0.001),GATAa(0.64,P<0.01)and DefC(0.28,P<0.05)were significantly down-regulated.Conclusions The concordance of Ae.albopictus with Ae.aegypti was higher than that with An.gambiae.DOMEb,Ago3,Rel2b and DefC,can serve as the preferred target genes for subsequent studies on DENV2 immune blockade.关键词
先天免疫/白纹伊蚊/登革病毒Key words
Innate immunity/Aedes albopictus/Dengue virus分类
医药卫生引用本文复制引用
周洋,李满金,程进,邹亚明,朱丁,兰策介,沈元..白纹伊蚊感染DENV2免疫相关基因的筛选与分析[J].寄生虫与医学昆虫学报,2025,32(3):153-159,178,8.基金项目
江苏省新发重大传染病病原微生物医学重点实验室开放性课题(PM202407) （PM202407）
无锡市医学发展学科(FZXK2021010) （FZXK2021010）
