江西农业大学学报2025，Vol.47Issue(5)：1153-1160,8.DOI:10.3724/aauj.2025098
小鼠单倍体胚胎干细胞二倍化的研究
Research on diploidization of mouse haploid embryonic stem cells
摘要
Abstract
The normal pattern of mouse somatic cells is typically diploid,with all chromosomes existing in pairs.In 2011 and 2012,research teams successfully established mouse haploid embryonic stem cells derived from parthenogenetic and androgenetic origins,respectively.These cells possess only one set of chromosomes,amounting to half the number found in mouse somatic cells.This characteristic offers unique advantages in fields such as sequencing,genetic screening,and gene and chromosome editing.Androgenetic haploid cells,in particular,can generate fertile offspring via injection into MII oocytes,significantly enhancing the efficiency of gene editing in mice,especially for large-fragment edits.However,the proportion of haploid cells in mouse haploid embryonic stem cell lines inevitably declines and eventually disappears with prolonged culture time and increased passages.This phenomenon,observed in the study of haploid animal cells,has considerably limited the application and promotion of haploid cells due to the inconveniences it causes.Over the years,reseachers have conducted multidimensional research and exploration into the causes and solutions of this phenomenon,achieving certain progress.This article provides a brief review and summarize previous research findings in two aspects:(1)the mechanisms underlying diploidization in mouse haploid embryonic stem cells and the associated scientific questions,and(2)the methods to address diploidization.It aims to facilitate understanding of this field for future researchers and provide inspiration.关键词
小鼠胚胎干细胞/单倍体/单倍体二倍化/倍性变化/细胞周期Key words
mouse embryonic stem cells/haploid/haploid diploidization/ploidy variation/cell cycle分类
生物科学引用本文复制引用
周湛岚,李劲松..小鼠单倍体胚胎干细胞二倍化的研究[J].江西农业大学学报,2025,47(5):1153-1160,8.基金项目
国家自然科学基金项目(32488101)和中国科学院战略性先导科技专项(XDB0990000)Project supported by the National Natural Science Foundation of China(32488101)and the Priority Research Pro-gram of the Chinese Academy of Sciences(XDB0990000) （32488101）
