江西农业大学学报2025，Vol.47Issue(5)：1203-1213,11.DOI:10.3724/aauj.2025103
Mn3O4纳米拟酶清除活性氧维持钠钾稳态提高水稻耐盐性
Mn3O4 nanozymes increased rice salt tolerance via ROS scavenging and maintenance of Na+/K+homeostasis
摘要
Abstract
[Objective]This study aims to investigate the effects of manganese tetraoxide(Mn₃O₄)nanozymes on the regulation of reactive oxygen species(ROS)homeostasis and Na⁺/K⁺ ion homeostasis in rice under salt stress,thus providing a theoretical basis for the application of nanomaterials in crop salt tolerance.[Method]In this study,the indica-type three-line hybrid rice variety"Quanyou 737"was selected as the test material to perform an indoor experiment.Polyacrylic acid(PAA)-coated Mn₃O₄ nanozymes(PMO)were used as the test nanomaterial.Rice plants were treated with 200 mmol/L NaCl for salt stress.The ability of PMO in maintaining ROS homeostasis in rice plants under salt stress was comprehensively evaluated by measuring the contents of ROS such as hydrogen peroxide(H₂O₂)and superoxide anion(O₂•⁻),as well as the activities of antioxidant enzymes including SOD,CAT,and POD in rice leaves.The impacts of PMO on the regulation of ion homeostasis in rice were assessed by detecting Na⁺ and K⁺ contents and their ratio,combined with qRT-PCR analysis of the expression of related ion transporter genes.[Result]Rice seedlings treated with 200 mg/L PMO exhibited a better phenotype of rice seedlings than control,which have higher plant height and fresh/dry weight,indicating a significant improvement in growth status.After PMO treatment,the contents of H₂O₂ and O₂•⁻ in rice leaves decreased significantly by 20.6%and 18.4%,respectively.The activities of major antioxidant enzymes such as SOD,CAT,and POD were significantly enhanced,indicating increased ROS scavenging capacity and alleviated oxidative stress.Meanwhile,the Na⁺ content in rice leaves decreased by 16.9%,the K⁺ content increased by 20.5%,and the Na⁺/K⁺ ratio decreased significantly.The expression levels of ion transporter genes OsNHX1,OsSOS1,and OsGORK showed no significant difference compared to the control,while the expression of OsHKT and OsHAK genes was significantly upregulated,leading to a marked improvement in ion homeostasis.[Conclusion]Under salt stress,200 mg/L PMO significantly improved the salt tolerance of rice plants by maintaining ROS homeostasis and ion homeostasis.关键词
水稻/Mn3O4纳米拟酶/纳米生物技术/抗氧化酶/盐胁迫/活性氧稳态/钠钾稳态Key words
rice/Mn3 O4 nanozymes/nano-enabled biotechnology/antioxidant enzymes/salt stress/ROS homeostasis/Na+/K+homeostasis分类
农业科学引用本文复制引用
李燕辉,何晔,包恩慧,周宵,胡隽琪,翟书伟,吴洪洪..Mn3O4纳米拟酶清除活性氧维持钠钾稳态提高水稻耐盐性[J].江西农业大学学报,2025,47(5):1203-1213,11.基金项目
国家自然科学基金项目(32120103008)和湖北省重点研发计划项目(2024BBB065)Project supported by the National Natural Science Foundation of China(32120103008)and Hubei Key R&D Pro-gram Project(2024BBB065)湖北省大学生创新创业训练计划项目(S202410504020)以及华中农业大学徐雯颍女士、曹建波先生和何立敏女士在获取本研究TEM结果中提供了帮助,谨致谢意! （32120103008）
