江西农业大学学报2025，Vol.47Issue(5)：1245-1256,12.DOI:10.3724/aauj.2025107
感病前后栀子果微生物群落结构与多样性
Microbial community structure and diversity of fructus gardenia before and after infection
摘要
Abstract
[Objective]This study aims to investigate the microbial community structure variation between diseased and healthy Gardenia jasminoides fruits,and identify potential pathogens.[Method]Metagenome was employed to analyze microbial community structures in three types of infected fruits versus healthy controls.Pathogen prediction was conducted through alignment with PHI and VFDB databases.[Result](1)The overall microbial community diversity of the three diseased Gardenia jasminoides fruit samples was lower than that of the healthy ones,However,the abundance and species number of the fungal community in them increased significantly,while the abundance ratio of bacterial to fungal communities(B/F)decreased.(2)At the fungal level,Ascomycota predominated in both diseased and healthy fruits.The relative abundance of Ramularia collo-cygni and Fusarium torreyae in scab(SC)samples was significantly higher than that in other samples.The relative abundance of Zasmidium and Talaromyces in dry rot(DR)samples was significantly higher than other samples.(3)At the bacteria level,Pseudomonadota was predominantly dominant in both infected and healthy fruits,and the Methylobacterium in the three diseased samples was relatively higher than that in the healthy samples.The samples of scab also contained high relative abundance of Brevibacillus parabrevis and Bacillus amyloliquefaciens.(4)Comparison with the Pathogen-Host Interaction Database(PHI-base)and the Virulence Factor Database of Pathogenic Bacteria(VFDB)revealed that Aspergillus fumigatus,Magnaporthe oryzae,Fusarium graminearum,Zymoseptoria tritici,Pantoea dispersa,and Pantoea dispersa B may be potential pathogens of Gardenia jasminoides fruit disease.[Conclusion]Fungal abundance elevation correlates with Gardenia fruit disease.R.collo-cygni and F.torreyae are potential scab pathogens,while Z.cellare may cause gardenia fruit dry rot.Aspergillus fumigatum,Macrophyllum oryzae,Fusarium graminis,Zymoseptoria tritici,Pantoea dispersa and Pantoea dispersa B may be the candidate pathogens of gardenia fruit disease.Brevibacillus parabrevis and Bacillus amyloliquefaciens may be involved in the biological control of Gardenia fruit diseases.关键词
栀子/宏基因组/病果/群落结构Key words
Gardenia jasminoids J.Ellis/metagenome/diseased fruit/community structure分类
农业科技引用本文复制引用
于丽欣,李康琴,卢燕萍,谢雄雄,邓绍勇..感病前后栀子果微生物群落结构与多样性[J].江西农业大学学报,2025,47(5):1245-1256,12.基金项目
国家自然科学基金项目(32060356)、江西省"科技+水利"联合计划项目(2022KSG01001)和中央财政林业科技推广示范项目(JXTG[2022]12号)Project supported by the National Natural Science Foundation Project(32060356),Jiangxi Provincial Joint-plan Project of Science and Technology&Water Conservancy(2022KSG01001)and Central Fiscal Forestry Science and Technology Promotion Demonstration Project(JXTG[2022]12)江西省林业科学院基础研究与科技成果转化专项(2025522803)同时对本研究给予了资助,谨致谢意! （32060356）
