江西农业大学学报2025，Vol.47Issue(5)：1383-1393,11.DOI:10.3724/aauj.2025119
低蛋白日粮条件下丝氨酸对miR-143缺失小鼠生长性能与脂肪肌肉代谢的影响
Effect of serine on growth performance and adipose-muscle metabolism in miR-143-deficient mice in low-protein diet
摘要
Abstract
[Objective]This study aims to investigate the effects of serine on growth performance and fat-muscle metabolism in miR-143-deficient mice under low-protein diet conditions,thus providing new scientific evidence for revealing the interaction between serine and miR-143.[Method]Twenty 4-week-old wild-type(WT)mice and 20 miR-143 knockout(KO)mice with similar body weights were randomly selected.Each type of mouse was further randomly divided into 2 groups,receiving normal drinking water or drinking water supplemented with 1％serine respectively.The effects of serine on growth performance,body composition,grip strength,weights of fat and muscle tissues,and hemoglobin content in miR-143-deficient mice were determined and analyzed.[Result]Under the low-protein diet conditions:(1)compared with WT mice,miR-143 deficiency significantly decreased feed intake and body weight of mice(P<0.05);compared with the normal drinking water group,supplementation of 1％serine in drinking water significantly reduced the body weight of WT mice(P<0.05)but had no significant effect on miR-143-deficient mice.(2)Compared with WT mice,miR-143-deficient mice showed an extremely significant decreases in lean meat weight,fat weight,and fat content(P<0.01),and a significant increase in lean meat content(P<0.05);compared with the normal drinking water group,1％serine supplementation showed an extremely significant fat weight and content decrease(P<0.01)and significantly increased lean meat content(P<0.05)in WT mice,but had no significant effect on fat content in miR-143-deficient mice.(3)Compared with WT mice,the deletion of miR-143 extremely significantly reduced the grip strength of mice(P<0.01);while compared with the normal drinking water group,the addition of 1％serine showed a tendency to decrease the grip strength of WT mice(P=0.125),and had no significant effect on the grip strength of miR-143-deficient mice.(4)Compared with WT mice,miR-143-deficient mice exhibited extremely significant decreases in the weights of liver,subcutaneous fat,epididymal fat,and brown fat,as well as the contents of subcutaneous fat,epididymal fat,and brown fat(P<0.01);compared with the normal drinking water group,1％serine supplementation significantly reduced the liver and subcutaneous fat weights,and the contents of subcutaneous fat,epididymal fat,and brown fat in WT mice(P<0.05),but had no significant effect on these indices in miR-143-deficient mice.(5)Compared with WT mice,miR-143-deficient mice had an extremely significant increase in gastrocnemius muscle content(P<0.01),significant increases in soleus muscle,tibialis anterior muscle,and extensor digitorum longus muscle contents(P<0.05),and a significant decrease in tibialis anterior muscle weight(P<0.05);compared with the normal drinking water group,1％serine supplementation extremely and significantly increased gastrocnemius muscle content(P<0.01)and significantly increased soleus muscle content(P<0.05)in WT mice,with tendencies to increase tibialis anterior muscle(P=0.108)and extensor digitorum longus muscle contents(P=0.106);however,in miR-143-deficient mice,it extremely and significantly reduced the weights of gastrocnemius muscle,soleus muscle,and tibialis anterior muscle(P<0.01)and significantly increased gastrocnemius muscle content(P<0.05).(6)miR-143 deficiency extremely and significantly increased the hemoglobin content of mice(P<0.01),while 1％serine supplementation had no significant effect on hemoglobin content(P>0.05).[Conclusion]Both 1％serine supplementation and miR-143 deficiency reduce mouse body weight,fat weight,fat content,and grip strength,and increase the contents of gastrocnemius muscle,soleus muscle,tibialis anterior muscle,and extensor digitorum longus muscle.However,after miR-143 deficiency,serine had no significant effect,indicating a preliminary interaction between serine and miR-143.关键词
丝氨酸/miR-143/脂肪代谢/肌纤维Key words
serine/miR-143/lipometabolism/muscle fiber分类
农业科技引用本文复制引用
陈星平,云冰,陈佳,罗君谊,陈婷,孙加节,张永亮,习欠云..低蛋白日粮条件下丝氨酸对miR-143缺失小鼠生长性能与脂肪肌肉代谢的影响[J].江西农业大学学报,2025,47(5):1383-1393,11.基金项目
国家自然科学基金项目(32402709)和江西省自然科学基金项目(20232BAB215052)Project supported by the National Natural Science Foundation of China(32402709)and Jiangxi Provincial Natural Science Foundation and the Science(20232BAB215052)江西省教育厅科学技术研究项目(GJJ2200413)同时对本研究给予了资助,谨致谢意! （32402709）
