解放军医学院学报2025，Vol.46Issue(7)：680-689,698,11.DOI:10.12435/j.issn.2095-5227.24100803
基于11.7 T磁共振探索超短波对轻度创伤性脑损伤大鼠脑灰质体积的影响
Effects of ultra short wave therapy on gray matter volume in rat models of mild traumatic brain injury using 11.7 T MRI
马珩钞 1陈桂琳 1杨澳娇 1史旭东 1倪立桐 2宁亚蕾 3江小霞 4李世俊 5张军6
作者信息
- 1. 解放军医学院,北京 100853||解放军总医院第一医学中心神经外科,北京 100853
- 2. 解放军医学院,北京 100853||解放军总医院第一医学中心放射诊断科,北京 100853
- 3. 陆军军医大学陆军特色医学中心,重庆 400038
- 4. 军事科学院军事医学研究院,北京 100850
- 5. 解放军总医院第一医学中心放射诊断科,北京 100853
- 6. 解放军总医院第一医学中心神经外科,北京 100853
- 折叠
摘要
Abstract
Background Mild traumatic brain injury(mTBI)affects approximately 42 million individuals annually,constituting a major global public health challenge.The 11.7T ultrahigh-field magnetic resonance imaging(MRI)offers novel strategies for tracking microstructural dynamics and elucidating repair mechanisms in mTBI.Objective To investigate the effects of ultrashort wave(USW)intervention on dynamic gray matter volume(GMV)changes and neurobehavioral functions in mTBI rats using 11.7T MRI.Methods Forty-five male Sprague-Dawley rats were randomly divided into mTBI group(single blast wave-induced mTBI model),USW group(mTBI with USW intervention),and control(Ctrl)group(no injury or intervention),with 15 rats in each group.Longitudinal 11.7T MRI T2-weighted imaging was performed during acute(3-5 days),subacute(2 weeks),and chronic(1 month)phases.Voxel-based morphometry(VBM)was applied to analyze whole-brain GMV alterations,while open field test(OFT)and elevated plus maze(EPM)were synchronously conducted to quantify locomotor activity and anxiety-like behaviors.Results Compared to the Ctrl group,the mTBI group exhibited significantly reduced GMV in 11,5,and 4 clusters at 3-5 days,2 weeks,and 1 month,respectively(P＜0.05),predominantly involving the prelimbic system,striatum,and parietal associative cortex.USW intervention markedly attenuated GMV atrophy,with 4/6,3/4,and 2/3 clusters showing GMV recovery at the corresponding time points(P＜0.05).Longitudinal analysis revealed gradual GMV recovery in the mTBI group over 1 month,whereas the USW group achieved stable GMV levels by 2 weeks.The mTBI group displayed acute locomotor deficits(increased%slow movement time vs Ctrl,P＜0.01)and persistent anxiety-like behaviors(reduced open-arm activity vs Ctrl,P＜0.05).In contrast,the USW group showed significant improvements in locomotor function and anxiety-like responses by 2 weeks(increased total distance and open-arm activity,P＜0.05),with anxiety indices normalizing to Ctrl levels at 1 month(P＞0.05),while the mTBI group retained chronic anxiety(reduced open-arm entries,P＜0.05).Correlation analysis identified significant associations between GMV reductions in the right prelimbic system(r=-0.631)and striatum(r=-0.645)with increased%slow-motion time,and between left parietal associative cortex GMV loss(r=0.727)and reduced open-arm time(P＜0.05).Conclusion VBM analysis based on 11.7T MRI sensitively captures dynamic GMV evolution post-mTBI.USW intervention promotes GMV restoration,mitigates acute motor deficits,and alleviates chronic anxiety-like behaviors,thus providing dual imaging and behavioral evidence to support precision rehabilitation strategies for mTBI.关键词
创伤性脑损伤/超高场强磁共振/基于体素的形态学测量/超短波/行为学评估/大鼠Key words
mild traumatic brain injury/ultra-high field magnetic resonance imaging/voxel-based morphometry/ultrashort wave/behavioral assessment/rat分类
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马珩钞,陈桂琳,杨澳娇,史旭东,倪立桐,宁亚蕾,江小霞,李世俊,张军..基于11.7 T磁共振探索超短波对轻度创伤性脑损伤大鼠脑灰质体积的影响[J].解放军医学院学报,2025,46(7):680-689,698,11.
