解放军医学院学报2025，Vol.46Issue(7)：699-703,5.DOI:10.12435/j.issn.2095-5227.25040201
维生素K2对高脂饮食小鼠胰岛素抵抗的影响及机制研究
Effects of vitamin K2 on insulin resistance of mice fed on high fat diet and related mechanisms
摘要
Abstract
Background Insulin resistance is a key factor in the pathogenesis of type 2 diabetes mellitus.Vitamin K2 can improve insulin sensitivity,but its underlying mechanism remains unclear.Objective To investigate the effects of vitamin K2 on insulin resistance of mice fed on a high fat diet and related mechanisms.Methods Aged mice were divided into normal diet group(a standard diet and olive oil oral gavage),HFD group(high fat diet,with olive oil oral gavage),and HFD-VK group(high fat diet,with 120 mg/kg[wt/wt]vitamin K2 dissolved in equivalent olive oil oral gavage).Body weight was recorded every week.Fasting blood glucose and insulin were detected to evaluate HOMA-IR.Intraperitoneal glucose tolerance tests(IPGTT)and intraperitoneal insulin tolerance test(IPITT)were used to examine the glucose disposal ability,HE staining was used to examine morphology of liver and white adipose tissue,Western blotting was used to investigate the protein levels of p-AMPK in liver and white adipose tissue.Results Compared with the HFD group,vitamin K2 administration significantly reduced body weight,decreased glucose and insulin in blood and thus exhibited an improvement in HOMA-IR;it increased glucose tolerance and insulin sensitivity and so improved glucose disposal ability(P＜0.05);and it could also improve morphology and activate AMPK in the liver and white adipose tissue(P＜0.05).Conclusion Vitamin K2 attenuates insulin resistance of mice fed on a high fat diet,which is related in activating AMPK in the liver and adipose tissue.关键词
维生素K2/肥胖/2型糖尿病/胰岛素抵抗/AMP活化蛋白激酶/小鼠Key words
vitamin K2/obesity/type 2 diabetes/insulin resistance/AMPK/mice分类
医药卫生引用本文复制引用
罗艺,汪菲,霍文静,刘姗姗,韩冰清,曾强..维生素K2对高脂饮食小鼠胰岛素抵抗的影响及机制研究[J].解放军医学院学报,2025,46(7):699-703,5.基金项目
国家自然科学基金项目(NSFC81872920 （）
NSFC82271770) （）
国家老年疾病临床医学研究中心项目(NCRCG-PLAGH-2024002) （NCRCG-PLAGH-2024002）
