精准医学杂志2025，Vol.40Issue(5)：391-397,7.DOI:10.13362/j.jpmed.202540100
miR155对阿霉素诱导的树突状细胞激活以及T细胞增殖的影响及其机制
Effect of miR155 on doxorubicin-induced activation of dendritic cells and T-cell proliferation and its mechanism
摘要
Abstract
Objective To investigate the effect of miR155 on doxorubicin(DOX)-induced dendritic cell(DC)activation and T-cell proliferation and its mechanism.Methods 4T1 cells cultured for 24 h in the cell culture medium containing 0,0.1,0.5,1.0,2.0,4.0,8.0,or 10.0 mg/L DOX(groups A-H),and the 4T1 cells transfected with miR155 were cultured for 24 h in the same cell culture medium(groups I-P).MTT assay was used to measure cell viability and calculate half-maximal inhibitory concentration(IC50).4T1 cells were divided into group Q(blank control group),group R(transfected with miR155),group S(transfected with miR155 and treated with 1 mg/L DOX),and group T(treated with 1 mg/L DOX).Flow cytometry was used to measure the fluorescence intensity of calreticulin(CRT)in 4T1 cells;ELISA and bioluminescence assay were used to measure the concentrations of high-mobility group box 1(HMGB1)and adenosine triphosphate(ATP)in supernatant;flow cytometry was used to measure the percentages of CD1 1c+CD80+CD86+cells and CD11c+MHCII+cells and the proliferation rate of CD3+T cells.Results MTT assay showed that DOX had an IC50 of 0.95 mg/L for 4T1 cells at 24 hours,and the addition of miR155 did not significantly enhance or attenuate the effect of DOX(P＞0.05).There were no significant differences in the fluorescence inten-sity of CRT in cells and the concentrations of ATP and HMGB1 in cell supernatant between group Q and group R(P＞0.05).Com-pared with group Q,group S had significant increases in the percentages of CD11c+CD80+CD86+cells and CD11c+MHCⅡ+cells and the proliferation rate of T cells(F=116.28-593.65,P＜0.05),and group R showed significantly higher values than group Q(F=54.45-174.27,P＜0.05).Conclusion This study shows that miR155 can enhance DOX-induced DC maturation,antigen presentation,and T-cell proliferation,and therefore,increasing the expression level of miR155 in 4T1 cells may enhance the thera-peutic effect of DOX.关键词
乳腺肿瘤/微RNAs/多柔比星/免疫疗法/树突细胞/肿瘤微环境/免疫原性细胞死亡Key words
Breast neoplasms/MicroRNAs/Doxorubicin/Immunotherapy/Dendritic cells/Tumor microenvironment/Immunogenic cell death分类
临床医学引用本文复制引用
江晓霞,李康康,沈洁,滕睿,张秀芹..miR155对阿霉素诱导的树突状细胞激活以及T细胞增殖的影响及其机制[J].精准医学杂志,2025,40(5):391-397,7.基金项目
山东省自然科学基金面上项目(ZR2021MH190) （ZR2021MH190）
