精准医学杂志2025，Vol.40Issue(5)：398-402,5.DOI:10.13362/j.jpmed.202540085
细胞外基质硬度对原代人前列腺癌细胞炎症因子表达的影响
Influence of extracellular matrix stiffness on the expression of inflammatory factors in primary hu-man prostate cancer cells
摘要
Abstract
Objective To investigate the influence of extracellular matrix(ECM)stiffness on the expression of inflam-matory factors in primary human prostate cancer cells.Methods Primary human prostate cancer cells were inoculated into cul-ture dishes pre-incubated with 0.1 g/L ECM solution(group A)or 8 g/L ECM solution(group B),and bright-field microscopy was used to observe cell morphology.On day 4 of culture,RT-qPCR and Western blot were used to measure the relative mRNA and protein expression levels of the inflammatory factors C-C motif chemokine ligand 2(CCL2),C-X-C motif chemokine ligand 10(CXCL10),intercellular adhesion molecule-1(ICAM1),and interleukin-32(IL-32)in cells.Results Bright-field microscopy showed that at 24 hours after inoculation,the cells in group A gradually aggregated and formed sheet-like structures,while those in group B exhibited multilayered cell aggregates.RT-qPCR showed that compared with group A,group B had significantly higher relative mRNA expression levels of the inflammatory factors CCL2,CXCL10,ICAM1,and IL-32(r=5.30-16.23,P＜0.05).Western blot showed that compared with group A,group B had significantly higher relative protein expression levels of ICAM1,IL-32,and CCL2(t=2.78-14.72,P＜0.05),while there was no significant difference in the relative protein expression level of CXCL10 between groups A and B(P＞0.05).Conclusion Soft ECM substrate can promote the expression of the inflammatory factors CCL2,CXCL10,ICAM1,and IL-32 in primary human prostate cancer cells.关键词
前列腺肿瘤/原代细胞培养/细胞外基质/硬度/细胞因子类Key words
Prostatic neoplasms/Primary cell culture/Extracellular matrix/Hardness/Cytokines分类
临床医学引用本文复制引用
李睿智,牟洁,祝海,李菁,王栋..细胞外基质硬度对原代人前列腺癌细胞炎症因子表达的影响[J].精准医学杂志,2025,40(5):398-402,5.基金项目
国家自然科学基金面上项目(82472967) （82472967）
山东省自然科学基金面上项目(ZR2023MH327) （ZR2023MH327）
青岛市自然科学基金原创探索项目(23-2-1-193-zyyd-jch) （23-2-1-193-zyyd-jch）
青岛大学"医学+"学科集群联合探索项目 （）
青岛市医疗卫生重点学科建设项目 （）
