精准医学杂志2025，Vol.40Issue(5)：435-440,6.DOI:10.13362/j.jpmed.202540093
抗癫痫药对成年癫痫患者体内细胞铁死亡的影响
Effect of antiepileptic drugs on ferroptosis of cells in adult patients with epilepsy
摘要
Abstract
Objective To investigate the effect of monotherapy with valproic acid(VPA),lamotrigine(LTG)or leveti-racetam(LEV)on ferroptosis of cells in adult patients with epilepsy.Methods A total of 95 patients with epilepsy who were treated in Department of Neurology,The Affiliated Hospital of Qingdao University,from December 2022 to September 2024 were enrolled as epilepsy group,and according to the type of the drug used,they were divided into VPA group,LTG group,and LEV group;93 individuals,matched for age and sex,who underwent physical examination in the same hospital during the same period of time were enrolled as control group.Fasting peripheral venous blood samples were collected from all subjects at baseline and from the patients with epilepsy after 6 months of medication.Colorimetry was used to measure the serum levels of ferrous ion(Fe2+),glutathione(GSH),and malondialdehyde(MDA),and RT-qPCR and Western blot(WB)were used to measure the ex-pression levels of glutathione peroxidase 4(GPX4)and solute carrier family 7 member 11(SLC7A11)in peripheral blood.The above indicators were compared between groups.Results At baseline,compared with the control group,the epilepsy group had significantly higher serum levels of Fe2+and MDA and significantly lower levels of GSH,GPX4,and SLC7A11(t=15.62-18.17,Z=-6.97-3.16,P＜0.05).After treatment,VPA,LTG,and LEV all significantly reduced the serum levels of Fe2+and MDA in the epilepsy group(t=5.14-14.75,P＜0.05),and LTG and VPA induced significantly greater reductions than LEV(F=5.94,7.05,t=-3.56--2.38,P＜0.05).VPA,LTG,and LEV all inhibited the downregulation of GSH,GPX4,and SLC7A11 in pe-ripheral blood(t=3.06-11.15,P＜0.05),with no significant difference in the degree of such inhibition between the three drugs(P＞0.05).Conclusion The three antiepileptic drugs VPA,LTG,and LEV can inhibit ferroptosis of cells in adult patients with epilepsy,while LTG and VPA have a better effect than LEV in improving iron overload and lipid peroxidation during ferroptosis.关键词
癫痫/铁死亡/抗癫痫药/丙戊酸/拉莫三嗪/左乙拉西坦/治疗结果/成年人Key words
Epilepsy/Ferroptosis/Anticonvulsants/Valproic acid/Lamotrigine/Levetiracetam/Treatment outcome/Adult分类
临床医学引用本文复制引用
王飞宇,钟茹杰,杨海林,李啸宇,刘雪,高静..抗癫痫药对成年癫痫患者体内细胞铁死亡的影响[J].精准医学杂志,2025,40(5):435-440,6.基金项目
青岛大学附属医院临床医学+X科研面上项目(QDFY+X202101029) （QDFY+X202101029）
