Abstract
Objective To investigate the correlation of interleukin(IL)-6,interleukin-8(IL-8),tissue inhibitor of me-talloproteinase-1(TIMP-1),miRNA-128,and miRNA-381 with the clinical stage of lower extremity atherosclerotic occlusive di-sease(LEASO)in elderly patients.Methods A total of 167 patients with LEASO,aged ≥65 years,who were hospitalized in Department of Vascular Surgery,Qingdao Municipal Hospital,from January 2024 to January 2025 were enrolled,and according to the Fontaine stage,they were divided into group A(stage Ⅰ/Ⅱ)and group B(stage Ⅲ/Ⅳ).Baseline data were collected,inclu-ding sex,age,body mass index,disease duration,smoking history,comorbidities,and medication regimens;ELISA and RT-qPCR were used to measure the serum levels of IL-6,IL-8,and TIMP-1 and the relative expression levels of rniRNA-128 and miRNA-381;a point-biserial correlation analysis was used to investigate the correlation of the serum levels of miRNAs and inflam-matory factors with clinical stage;the logistic regression analysis was used to investigate the influencing factors for the clinical pro-gression of LEASO.Results Compared with group A,group B had significantly higher serum levels of IL-6 and IL-8(Z=3.074,7.354,P＜0.05),a significantly lower serum level of TIMP-1(Z=-8.031,P＜0.05),and significantly lower relative ex-pression levels of miRNA-128 and miRNA-381 in serum(Z=-2.882,-3.164,P＜0.05).The point-biserial correlation analysis showed that the serum levels of IL-6 and IL-8 were positively correlated with clinical stage(r=0.154,0.557,P＜0.05),and the levels of TIMP-1,miRNA-128,and miRNA-381 in serum were negatively correlated with clinical stage(r=-0.617,-0.251,-0.253,P＜0.05).The multivariate logistic regression analysis showed that an increase in the serum level of IL-8(OR=1.069,95％CI=1.025-1.115,P＜0.05),a reduction in TIMP-1(OR=0.961,95％CI=0.935-0.987,P＜0.05),a reduction in miRNA-381(OR=11.674,95％CI=1.678-81.228,P＜0.05),and prolonged disease duration(OR=2.669,95％CI=1.744-4.087,P＜0.05)were risk factors for the clinical progression of LEASO.Conclusion The serum levels of IL-8,TIMP-1,and miRNA-381 are correlated with the clinical stage of LEASO and may be used as potential biomarkers for assessing the severity of LEASO.关键词
闭塞性动脉硬化/下肢/微RNAs/白细胞介素6/白细胞介素8/金属蛋白酶1组织抑制剂Key words
Arteriosclerosis obliterans/Lower extremity/MicroRNAs/Interleukin-6/Interleukin-8/Tissue inhibitor of metal-loproteinase-1分类
临床医学
