昆明医科大学学报2025，Vol.46Issue(11)：26-34,9.DOI:10.12259/j.issn.2095-610X.S20251104
基于SPOP/HnRNPK通路探讨双氢青蒿素对前列腺癌增殖和转移的影响
Investigating the Effect of Dihydroartemisinin on Proliferation and Metastasis of Prostate Cancer Cells based on the SPOP/HnRNPK Pathway
摘要
Abstract
Objective To investigate the effects of dihydroartemisinin(DHA)on the proliferation and metastasis of prostate cancer cells via the speckle-type POZ protein(SPOP)/heterogeneous nuclear ribonucleoprotein K(HnRNPK)pathway.Methods The prostate cancer cell line DU145 was cultured in vitro and treated with different concentrations of DHA.Cells were divided into groups based on DHA concentration:blank group(0 μmol/L),low-concentration group(10 μmol/L),medium-concentration group(50 μmol/L),and high-concentration group(100 μmol/L).Colony formation assay,scratch assay,and Transwell assay were performed to observe changes in proliferation,migration,and invasion of DU145 cells under different DHA treatments.Western blotting was used to detect the expression levels of SPOP,HnRNPK,Vimentin,Neural-cadherin(N-cadherin),Epithelial-cadherin(E-cadherin),and Zonula occludens-1(ZO-1).SPOP expression was knocked down in DU145 cells(SPOP-shNC-DU145,SPOP-sh-DU145),which were then subjected to the aforementioned low,medium,and high DHA concentration interventions for rescue experiments.A xenograft mouse model was established by injecting SPOP-knockdown cells into mice,followed by oral administration of low(2.5 mg/kg),medium(5 mg/kg),and high(10 mg/kg)concentrations of DHA.Results Compared with the blank group,the proliferation,migration,and invasion of DU145 cells in the low-,medium-,and high-concentration groups were significantly inhibited(P<0.05),Intracellular expression levels of HnRNPK,Vimentin,and N-cadherin were decreased(P<0.05),while the expression levels of SPOP,E-cadherin,and ZO-1 were increased(P<0.05).Compared with SPOP-shNC DU145 cells,the inhibitory effects of DHA on SPOP-sh DU145 cells were attenuated,with increased expression levels of HnRNPK,Vimentin,and N-cadherin(P<0.05),and decreased expression levels of E-cadherin and ZO-1(P<0.05).Conclusion DHA may target and induce increased SPOP expression,thereby inhibiting the proliferation and metastasis of DU145 cells.关键词
斑点型POZ蛋白/核内不均一核糖核蛋白K/双氢青蒿素/前列腺癌Key words
Speckle-type POZ protein/Heterogeneous nuclear ribonucleoprotein K/Dihydroartemisinin/Prostate cancer分类
医药卫生引用本文复制引用
张鉴通,高振庄,王之秀,孙丽娜..基于SPOP/HnRNPK通路探讨双氢青蒿素对前列腺癌增殖和转移的影响[J].昆明医科大学学报,2025,46(11):26-34,9.基金项目
河北省卫生健康委员会医学科学研究课题(20241203) （20241203）
保定市科技计划项目(2441ZF227) （2441ZF227）
