昆明医科大学学报2025，Vol.46Issue(11)：58-64,7.DOI:10.12259/j.issn.2095-610X.S20251108
T细胞免疫结构蛋白促进Hippo通路在子痫前期发病中的作用机制
Mechanism by Which T Cell Immune Structural Proteins Promote the Role of the Hippo Pathway in the Pathogenesis of Preeclampsia
摘要
Abstract
Objective To explore the mechanism by which T cell immunoglobulin and mucin domain-3(TIM-3)promotes the Hippo signaling pathway in the pathogenesis of preeclampsia.Methods HTR-8/Svneo cells were divided into a control group(Con),a recombinant human Tim-3 protein(Tim-3 Fc)group,a YAP1 inhibitor CA3 group and a Tim-3 Fc+CA3 group.Cell proliferation was analyzed by 5-ethynyl-2 '-deoxyuridine(EdU).Cell invasion and migration were evaluated by Transwell assay,and cell apoptosis was analyzed by terminal deoxynucleotidyl transferase dUTP nick end labeling(TUNEL)assay.Protein expression levels of TIM-3 and Hippo pathway components in HTR-8/SVneo cells were detected by Western Blot.Results Compared to the Con group,protein expression levels of TIM-3,YAP1 and TAZ were up-regulated in HTR-8/SVneo trophoblast cells of the TIM-3 Fc group(P<0.05).Compared to the TIM-3 Fc group,protein expression levels of YAP1 and TAZ were down-regulated in trophoblast cells of the TIM-3 Fc+CA3 group(P<0.05).Compared to the Con group,the rate of EdU positive cells in HTR-8/SVneo trophoblast cells in TIM-3 Fc group was increased significantly(P<0.05).Compared to the TIM-3 Fc group,the rate of EdU positive cells and the number of apoptotic cells in HTR-8/SVneo trophoblast cells of the TIM-3 Fc+CA3 group were decreased significantly(P<0.05).Compared to the Con group,the number of HTR-8/SVneo trophoblast cells in TIM-3 Fc group was increased significantly(P<0.05).Compared to the TIM-3 Fc group,the number of HTR-8/SVneo trophoblast cells in TIM-3 Fc+CA3 group was decreased significantly(P<0.05).Conclusions Tim-3 activates the Hippo pathway by interacting with YAP1 in trophoblast cells,thus promoting cell proliferation,invasion and migration.关键词
T细胞免疫球蛋白结构域和粘蛋白结构域3/先兆子痫/Hippo通路/滋养层细胞Key words
T cell immunoglobulin and mucin domain-3/Preeclampsia/Hippo pathway/Trophoblast cell分类
医药卫生引用本文复制引用
张维一,杨丽萍,马建彩..T细胞免疫结构蛋白促进Hippo通路在子痫前期发病中的作用机制[J].昆明医科大学学报,2025,46(11):58-64,7.基金项目
邯郸市科学技术研究与发展计划项目(24422083016ZC) （24422083016ZC）
