临床口腔医学杂志2025，Vol.41Issue(11)：647-652,6.DOI:10.3969/j.issn.1003-1634.2025.11.003
基于SDF-1/CXCR4通路探讨牵张力对牙周膜成纤维细胞成骨分化的影响
Exploring the effect of stretch force on osteogenic differentiation of periodontal ligament fibroblasts based on SDF-1/CXCR4 pathway
摘要
Abstract
Objective:To investigate the effect and mechanism of stretch force(Stretch)on osteogenic differentiation of periodontal ligament fibroblasts(PDLFs).Methods:The hPDLFs were cultured in vitro and separated into Control group,stretch force intervention for 6 hours group(Stretch 6 h group),stretch force intervention for 12 hours group(Stretch 12 h group),SDF-1/CXCR4 pathway activator group(CTCE-0214 group),and stretch force intervention for 12 hours+SDF-1/CXCR4 pathway inhibitor group(Stretch 12 h+AMD3100 group).The FlexCell system was used to apply periodic stretch force to hPDLFs.Colorimetric and ALP staining methods were used to detect ALP activity in hPDLFs.Alizarin red staining was performed to observe the condition of calcified nodules.RT-qPCR method was used to detect the expression levels of os-teogenic related genes.Immunohistochemistry was performed to detect positive expression of BSP and COL1A1.Western blot was performed to detect the SDF-1/CXCR4 pathway proteins.Results:Compared with the Control group,the Stretch 6 h group,Stretch 12 h group,and CTCE-0214 group showed enhanced ALP activity and increased calcified nodules in hPDLFs.The relative expression levels of RUNX2,OPN,and OCN mRNA were increased,and the positive expression of BSP,COL1A1,and the expression of SDF-1/CXCR4 pathway proteins were also increased.Moreover,the osteogenic differentiation ability of hPDLFs in the Stretch 12 h group was higher than that in the Stretch 6 h group(P＜0.05).Compared with the Stretch 12 h group,the Stretch 12 h+AMD3100 group showed a decrease in hPDLFs ALP activity and a reduction in calcified nodules.The relative expression levels of RUNX2,OPN,and OCN mRNA were decreased,and the positive expression of BSP,COL1A1,and the expression of SDF-1/CXCR4 pathway proteins were also decreased(P＜0.05).Conclusion:Applying periodic stretch force can promote osteogenic differentiation of hPDLFs and accelerate orthodontic progression by activating the SDF-1/CXCR4 pathway.关键词
牵张力/牙周膜成纤维细胞/成骨分化/SDF-1/CXCR4信号通路Key words
Stretch force/Periodontal ligament fibroblasts/Osteogenic differentiation/SDF-1/CXCR4 signaling pathway分类
生物工程引用本文复制引用
张帅,韩桂贤,于向前,马青,黄亭..基于SDF-1/CXCR4通路探讨牵张力对牙周膜成纤维细胞成骨分化的影响[J].临床口腔医学杂志,2025,41(11):647-652,6.基金项目
2025年度河北省医学科学研究课题(20250907) （20250907）
张家口市重点研发计划项目(2421101D) （2421101D）
