临床口腔医学杂志2025，Vol.41Issue(11)：653-657,5.DOI:10.3969/j.issn.1003-1634.2025.11.004
3D生物打印GelMA/Bio-Oss/BMSCs复合支架修复犬下颌骨缺损研究
3D bioprinted GelMA/Bio-Oss/BMSCs composite scaffold study on repairing canine mandibular defects
摘要
Abstract
Objective:Evaluate the osteogenic efficacy of a 3D bioprinted composite scaffold composed of methacry-lated gelatin(GelMA)/bovine bone substitute(Bio-Oss)/bone marrow-derived mesenchymal stem cells(BMSCs)in repairing mandibular defects in Beagle dogs,providing a basis for developing novel bone regenerative materials.Methods:Mandibular defect models were established in the mandibles of 4 Beagle dogs(total of 24 defects).Based on the type of implanted materi-al,the dogs were randomly divided into four groups:Group A(implanted with GelMA/Bio-Oss/BMSCs composite scaffold),Group B(implanted with GelMA/Bio-Oss scaffold),Group C(blank control,only modeling without intervention),and Group D(positive control,implanted with Bio-Oss bone powder covered with Bio-Gide collagen membrane).At 4 and 12 weeks post-surgery,bone volume fraction(BV/TV)and bone mineral density(BMD)were analyzed using Micro-CT,and histological stai-ning(HE and Masson)was performed.Results:Micro-CT examination:Bone defect repair in Group A was significantly better than in Groups B and C(P＜0.05).Compared with Group D,Group A exhibited faster degradation.Histological examination:Group A showed active new bone regeneration,and the bone quality was more mature compared to Group D.Histological quantitative analysis:Compared to Groups B and C,Group A had a significantly increased percentage of collagen fiber area(P＜0.05).Conclusion:After implanting the GelMA/Bio-Oss/BMSCs composite scaffold into the mandibular defect of Bea-gle dogs,new bone tissue was stably formed,demonstrating good biocompatibility and osteogenic effects.关键词
骨髓间充质干细胞/甲基丙烯酰化明胶/3D生物打印/骨缺损修复/比格犬Key words
Bone marrow mesenchymal stem cells/Methyl acrylate gelatin/3D bioprinting/Bone defect repair/Bea-gle dogs分类
口腔医学引用本文复制引用
刘巧,刘蓉,陈梦洁,贺昊,李哲争,谢雨朦,朱丽雷..3D生物打印GelMA/Bio-Oss/BMSCs复合支架修复犬下颌骨缺损研究[J].临床口腔医学杂志,2025,41(11):653-657,5.基金项目
湖南省自然科学基金项目(2024JJ9538) （2024JJ9538）
长沙市自然科学基金项目(kq2208486) （kq2208486）
湖南中医药大学校级科研基金项目(2022XYLH130) （2022XYLH130）
