量子电子学报2025，Vol.42Issue(6)：750-758,9.DOI:10.3969/j.issn.1007-5461.2025.06.002
基于表面增强拉曼光谱测量抗磷脂酶A2受体抗体
Measurement of anti-phospholipase A2 receptor antibodies based on surface-enhanced Raman spectroscopy
张钰 1程蕊 1赵畅 1杨荟楠 1沈剑箫 2戚超君 2陈军1
作者信息
- 1. 上海理工大学能源与动力工程学院,上海 200093
- 2. 上海交通大学医学院附属仁济医院肾脏科,上海 200127
- 折叠
摘要
Abstract
Based on the self-developed surface enhanced Raman spectroscopy(SERS)test platform,the anti-phospholipase A2 receptor(anti-PLA2R)antibody aqueous solutions with varying titers were detected by employing a self-made nanosilver colloidal solution as the enhancement medium.It was found that under the enhancment effect of the nanosilver colloidal solution,all anti-PLA2R antibody aqueous solutions with different titers exhibited a characteristic peak at a Raman frequency shift of approximately 1521 cm-1,and the detection limit could reach 2 RU/mL.On this basis,further detection and analysis were conducted on urine samples from healthy individuals and urine samples mixed anti-PLA2R antibody aqueous solutions.It was found that only the samples mixed anti-PLA2R antibody aqueous solutions could exhibit the Raman characteristic peak at 1521 cm-1.This study indicates that the Raman characteristic peaks of PLA2R antibody can be detected by SERS,and SERS-based urine detection has promising potential as a novel method for clinically non-invasive diagnosis of idiopathic membranous nephropathy and can be served as an important guidance for whether suspected patients with idiopathic membranous nephropathy need detailed kidney tissue biopsy or not.关键词
光谱/拉曼特征峰/表面增强拉曼光谱/抗磷脂酶A2受体抗体/诊断Key words
spectroscopy/Raman characteristic peak/surface-enhanced Raman spectroscopy/anti-phospholipase A2 receptor(anti-PLA2R)antibody/diagnosis分类
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张钰,程蕊,赵畅,杨荟楠,沈剑箫,戚超君,陈军..基于表面增强拉曼光谱测量抗磷脂酶A2受体抗体[J].量子电子学报,2025,42(6):750-758,9.
