西南医科大学学报2025，Vol.48Issue(6)：603-610,8.DOI:10.3969/j.issn.2096-3351.2025.06.008
ZIF-8纳米粒的制备及其对雷公藤红素的载药性能优化
Preparation of ZIF-8 Nanoparticles and Optimization of Their Drug Loading Performance toward Celastrol
摘要
Abstract
Objective The aim of this study was to prepare a ZIF-8@Celastrol(ZIF-8@Cel)nano-drug delivery system using 2-methylimidazole and zinc nitrate hexahydrate as raw materials,and to enhance its encapsulation efficiency and drug loading capacity through process optimization,thereby improving the bioavailability of celastrol(Cel).Methods ZIF-8@Cel nanoparticles were syn-thesized using a two-step method:firstly,ZIF-8 was prepared via a room-temperature stirring method,and the synthesis process was optimized using single-factor experiments to determine the optimal preparation parameters;subsequently,the formulation and process conditions were screened via single-factor experiments to construct the ZIF-8@Cel nanocomposite system.The particle size,polydis-persity index(PDI),and Zeta potential of ZIF-8@Cel were determined by dynamic light scattering(DLS);structural characterization was performed using Fourier transform infrared spectroscopy(FT-IR)and ultraviolet-visible spectrophotometry(UV-vis);its mor-phology and size were observed by transmission electron microscopy(TEM);the encapsulation efficiency and drug loading were mea-sured by UV-vis;finally,in vitro release experiments were conducted in different pH release media to evaluate its drug release profile.Results The optimized ZIF-8@Cel nanoparticles had a particle size of approximately 100 nm,uniform size,and a regular rhombic dodecahedron morphology.The encapsulation efficiency of Cel was 81.14%,and the drug loading was 16.86%.The nano-system exhib-ited good stability over 7 days and demonstrated significant pH-responsive release characteristics:the cumulative release reached 81.82%at pH 5.4 within 48 hours,while it was only 33.26%at pH 7.4.Conclusion A pH-responsive ZIF-8@Cel nano-drug deliv-ery system was successfully constructed.It possesses a small particle size,uniform distribution,good encapsulation efficiency,and drug loading capacity,which can effectively improve the bioavailability of the poorly soluble drug Cel.This study provides a prelimi-nary foundation for the application of ZIF-8 in the delivery of natural active ingredients.关键词
金属有机框架材料/沸石咪唑酯骨架-8/雷公藤红素/纳米系统/载药性能优化Key words
Metal-Organic framework/Zeolitic imidazolate framework-8/Celastrol/Nano system/Drug loading optimization分类
临床医学引用本文复制引用
唐俊,梁丽娟,梁晓亚,刘怡琳,丁可伊,谭笑,李春红,李尧..ZIF-8纳米粒的制备及其对雷公藤红素的载药性能优化[J].西南医科大学学报,2025,48(6):603-610,8.基金项目
国家自然科学基金(82574332) （82574332）
泸州市人民政府-西南医科大学科技战略合作项目(2024LZXNYDJ070) （2024LZXNYDJ070）
合江县人民医院-西南医科大学战略合作项目(2023HJXNYD03) （2023HJXNYD03）
