农业环境科学学报2025，Vol.44Issue(10)：2499-2507,9.DOI:10.11654/jaes.2024-0929
基于代谢组学研究乙草胺对上海青生理代谢影响
Metabolomics reveals the effects of pesticide acetochlor on the physiological metabolism of Chinese cabbage
摘要
Abstract
To study the impact of acetochlor on the physiological metabolism of the Chinese cabbage,in this study,we examined the impact of acetochlor with various doses on the physiological changes and metabolite differences of Chinese cabbage through physiological and biochemical indices,as well as metabolomics analysis.The findings revealed that both low(5 mg·L-1)and high(50 mg·L-1)doses of acetochlor induced oxidative stress in Chinese cabbage,evidenced by a significant increase in malondialdehyde levels and the activities of superoxide dismutase and peroxidase.The high dose notably inhibited photosynthesis(chlorophyll content decrease)and total protein synthesis,ultimately leading to growth inhibition in Chinese cabbage.Metabolomics analysis identified 148 significantly altered metabolites.Linoleic acid and linolenic acid,which are crucial for maintaining cell membrane fluidity and regulating osmotic pressure,were significantly accumulated in both the high-and low-dose groups.In the low-dose group,there was a notable accumulation of defense-related amino acids,flavonoids,and phenolic acids in the leaves,which supported the plant defense response to stress.Conversely,these compounds were significantly decreased in the high-dose group,potentially impairing the plant adaptability to pesticides.Furthermore,the high dose of acetochlor significantly impacted the plant carbon metabolic pathway,leading to a marked reduction in the levels of most organic acids and lysophosphatidylcholine,likely associated with decreased photosynthesis.It was shown that high and low concentrations of acetochlor caused different physiological responses in Chinese cabbage leaves,and Chinese cabbage initiated different metabolic defence mechanisms to different concentrations of acetochlor.关键词
代谢组学/除草剂/乙草胺/胁迫响应/代谢通路Key words
metabolomics/herbicide/acetochlor/stress response/metabolic pathway分类
环境科学引用本文复制引用
张凯玮,陈蒙,张雪,李越,董春村,杨晨烨,李勇,余向阳..基于代谢组学研究乙草胺对上海青生理代谢影响[J].农业环境科学学报,2025,44(10):2499-2507,9.基金项目
国家自然科学基金项目(32272583)National Natural Science Foundation of China(32272583) （32272583）
