海南热带海洋学院学报2025，Vol.32Issue(5)：130-140,11.DOI:10.13307/j.issn.2096-3122.2025.05.15
应用耳蜗注射重组腺病毒技术调控成年小鼠耳蜗毛细胞的再生
Regulating Regeneration of Cochlear Hair Cells in Adult Mice via Cochlear Injection of Recombinant Adenovirus
摘要
Abstract
The Wnt and Notch signaling pathways,which play crucial roles in hair cell development,are also piv-otal in hair cell regeneration.To explore techniques for regenerating adult mouse cochlear hair cells,cochleostomy was employed.Recombinant adenovirus was microinjected into the scala media of adult mouse cochleae to infect supporting cells and modulate the activity of intracellular Wnt and Notch signaling pathways.Results showed that adenovirus-mediated alterations in both Wnt and Notch signaling pathways induced regeneration of cochlear hair cells in adult mice.Compared to modulating a single pathway alone,simultaneously altering the activity of both Wnt and Notch pathways demonstrated significantly greater effects on hair cell regeneration and supporting cell proliferation.Further analysis con-firmed that supporting cells subjected to dual-pathway regulation could proliferate and regenerate hair cells expressing marker genes characteristic of mature hair cells.This technique offers an effective approach for investigating hearing im-pairments resulting from cochlear hair cell damage.关键词
耳蜗造口术/腺病毒转染技术/Notch通路/Wnt通路/成年小鼠/毛细胞再生Key words
cochleostomy/adenoviral transfection technology/Notch pathway/Wnt pathway/adult mice/hair cell re-generation分类
临床医学引用本文复制引用
张信文,赵汝霞,杨思远,牛巧花,翁梦露,曾少举..应用耳蜗注射重组腺病毒技术调控成年小鼠耳蜗毛细胞的再生[J].海南热带海洋学院学报,2025,32(5):130-140,11.基金项目
国家自然科学基金项目(31860269) （31860269）
海南省自然科学基金项目(319QN238) （319QN238）
