水产科技情报2025，Vol.52Issue(6)：349-354,6.DOI:10.16446/j.fsti.20230500105
异齿裂腹鱼原代肝细胞分离培养与鉴定
Isolation,culture and identification of primary hepatocytes from Schizothorax o'connori
摘要
Abstract
To establish the in vitro isolation and culture of primary hepatocytes from Schizothorax o'connori and explore their biological characteristics,the liver tissue of S.o'connori was digested by collagenase digestion,and the primary hepatocytes were obtained by density gradient centrifugation.The cell morphology was observed.The growth cycle of hepatocytes was detected by CCK-8 kit and the growth curve was plotted.The isolated and purified primary hepatocytes were identified by periodic acid-Schiff staining(PAS)and cytokeratin-18(CK-18)immuno-fluorescence staining.The results showed that the morphology of primary hepatocytes of S.o'connori were intact and adhered well.However,the 4th-generation cultured cells failed to adhere and grow after digestion.The fluo-rescence staining of PAS and CK-18 was positive,and the purity of hepatocytes was above 90%,indicating suc-cessful isolation of primary hepatocytes of S.o'connori.关键词
异齿裂腹鱼/原代肝细胞/分离培养/鉴定Key words
Schizothorax o'connori/primary hepatocytes/isolation and culture/identification引用本文复制引用
王亚玲,何辉,孙帅杰,于光晴,许会芬,李明,次珍,周建设..异齿裂腹鱼原代肝细胞分离培养与鉴定[J].水产科技情报,2025,52(6):349-354,6.基金项目
现代农业产业技术体系——特色淡水鱼(CARS-46). （CARS-46）
