山东农业科学2025，Vol.57Issue(10)：18-27,10.DOI:10.14083/j.issn.1001-4942.2025.10.002
小麦生理型雄性不育系差异表达基因筛选与表达模式分析
Screening and Expression Pattern Analysis of Differentially Expressed Genes in a Physiologically Male-Sterile Wheat Line
摘要
Abstract
To explore the molecular mechanisms of chemical hybridizing agent SQ-1-induced pollen abor-tion in wheat,this study was conducted using the wheat cultivar Xinong 1376 as experimental material.The RNA-seq technology was employed to perform transcriptome sequencing of wheat florets treated with 5 kg·hm-2 SQ-1(PHYMS-1376)and water(control,CK-1376)at 8 h,24 h and 5 d.Differentially expressed genes(DEGs)were screened,followed by GO functional annotation,KEGG pathway analysis,and expression pattern investigation.The results showed that a total of 2 827 DEGs were identified,including 1 799 significantly upreg-ulated genes and 1 028 significantly downregulated genes.GO enrichment analysis indicated that the DEGs were significantly enriched in the biological processes such as photosynthesis and DNA replication,the cellular com-ponents such as thylakoid membrane and cell wall,and the molecular functions mainly related to electron trans-fer activity and ATPase activity.KEGG pathway analysis indicated that the DEGs were mainly participated in the pathways such as starch and sucrose metabolism,glutathione metabolism and phenylpropanoid biosynthesis.The expression patterns of 16 DEGs obtained by qRT-PCR analysis were highly consistent with the transcriptome a-nalysis results(R2=0.985 6).In conclusion,SQ-1 might induce male sterility by disrupting key pathways such as reactive oxygen species metabolism,carbon source allocation and energy supply,ultimately leading to im-paired synthesis of pollen development-related substances.These findings could provide a theoretical basis for further exploration of the molecular mechanisms of SQ-1 and its application in wheat heterosis utilization.关键词
小麦/化学杂交剂SQ-1/雄性不育/转录组测序/差异表达基因/GO富集分析/KEGG通路分析Key words
Wheat/Chemical hybridizing agent SQ-1/Male sterility/Transcriptome sequencing/Differ-entially expressed genes/GO enrichment analysis/KEGG pathway analysis分类
农业科学引用本文复制引用
付亮,吴国丽,马华平,李洋,周思远,李永珍,李政,蒋志凯..小麦生理型雄性不育系差异表达基因筛选与表达模式分析[J].山东农业科学,2025,57(10):18-27,10.基金项目
河南省现代农业产业技术体系建设专项(HARS-22-01-Z3) （HARS-22-01-Z3）
国家小麦产业技术体系建设专项(CARS-3-2-35) （CARS-3-2-35）
河南省农业(小麦)良种联合攻关项目"高产多抗优质小麦新品种的选育及应用"(2022010103) （小麦）
