山东农业科学2025，Vol.57Issue(10)：28-35,8.DOI:10.14083/j.issn.1001-4942.2025.10.003
分子标记辅助选择Pigm基因改良水稻不育系泰丰A的稻瘟病抗性
Improving Blast Resistance of Rice CMS Line Taifeng A by Pigm-Based Marker-Assisted Selection Strategy
摘要
Abstract
To orderly improve the resistance of rice CMS line Taifeng A to rice blast disease,we selected the rice cultivar Gumei 4,known for harboring the broad-spectrum and durable blast resistance gene Pigm,as the donor parent,and employed the maintainer line Taifeng B and its sterile line Taifeng A as the recipient parents,to conduct molecular marker-assisted selection utilizing the selected codominant marker S29742 loca-ted within the Pigm gene.In the Gumei 4 genome,the marker S29742 yielded a 554-bp amplification product,contrasting with a 745-bp product observed in the genomes of Taifeng A and Taifeng B,indicating a pro-nounced polymorphism.By selecting with marker S29742 and integrating field and laboratory identification of resistance to rice blast seedling blight,we successfully screened out two improved sterile lines,Taifeng A-Pigm-1 and Taifeng A-Pigm-2,along with their respective maintainer lines,Taifeng B-Pigm-1 and Taifeng B-Pigm-2.Through evaluations of fertility,agronomic traits and grain quality,we ultimately identified the refined sterile line Taifeng A-Pigm-1,characterized by high resistance to blast disease,significant sterility,stable ste-rility,favorable outcrossing habits and superior agronomic attributes,accompanied by its compatible maintai-ner line Taifeng B-Pigm-1.The results established a foundation for the continued development of new hybrid rice combinations with broad-spectrum,high-level resistance to rice blast disease.关键词
泰丰A/细胞质雄性不育系/稻瘟病抗性/Pigm基因/分子标记辅助选择Key words
Taifeng A/Cytoplasmic male sterile(CMS)line/Rice blast resistance/Pigm gene/Marker assisted selection(MAS)分类
农业科技引用本文复制引用
邓吉奇,刘雄伦,黄俊,车凡昊,邹玉莹,周庚,郭成龙,谌强,李家鑫,刘金灵..分子标记辅助选择Pigm基因改良水稻不育系泰丰A的稻瘟病抗性[J].山东农业科学,2025,57(10):28-35,8.基金项目
湖南省科技重大专项(2021NK1001) （2021NK1001）
国家自然科学基金项目(31972256) （31972256）
