山东农业科学2025，Vol.57Issue(10)：36-41,6.DOI:10.14083/j.issn.1001-4942.2025.10.004
桑树韧皮部蛋白MaPP2基因的克隆及表达分析
Cloning and Expression Analysis of Mulberry MaPP2 Gene
摘要
Abstract
Phloem proteins participate in plant stress physiology and resistance.In order to explore the function of phloem protein 2(PP2)in mulberry,we cloned the MaPP2 gene of mulberry,and obtained a se-quence of 962 bp,which encoded 194 amino acids,and had three exons.Sequence analysis showed and the MaPP2 protein was a hydrophilic protein with molecular weight of 22.565 kDa and isoelectric point of 8.27.The MaPP2 had conservative domain of PP2 gene family,but had no signal peptide and transmembrane do-main.The results of amino acid sequence alignment and phylogenetic analysis showed that MaPP2 had high se-quence similarity and close phylogenetic relationship with the PP2 protein of Morus notabilis and Ficus carica.The qRT-PCR results showed that mulberry phytoplasma infection and MeJA could induce the expression of MaPP2 gene,while ABA and damage had inhibitory effects on its expression.The results of this study could provide a reference for further understanding the function and role of MaPP2 gene,as well as for the research of mulberry resistance.关键词
桑树/韧皮部蛋白2/表达分析/桑植原体侵染/非生物胁迫Key words
Mulberry/Phloem protein 2/Expression analysis/Mulberry phytoplasma infection/Abiotic stresses分类
农业科技引用本文复制引用
袁梦佳,陈静,杨金宏,孔卫青..桑树韧皮部蛋白MaPP2基因的克隆及表达分析[J].山东农业科学,2025,57(10):36-41,6.基金项目
陕西省教育厅科学研究计划重点项目(20JS003) （20JS003）
大学生创新创业训练计划项目(202211397011) （202211397011）
