山东农业科学2025，Vol.57Issue(10)：130-136,7.DOI:10.14083/j.issn.1001-4942.2025.10.016
融合杀虫蛋白M2CryAb-vip3A对非靶标生物龟纹瓢虫、日本通草蛉和白符跳的影响
Effects of Fusion Insecticidal Protein M2CryAb-vip3A on Non-Target Organisms Propylea japonica,Chrysoperla nipponensis and Folsomia candida
摘要
Abstract
Propylea japonica,Chrysoperla nipponensis and Folsomia candida are ecologically important non-target organisms that play a crucial role in maintaining ecological balance and biodiversity.M2CryAb-vip3A,a novel insect-resistant fusion protein designed by Shandong Academy of Agricultural Sciences,holds significant potential for agricultural pest control.To assess its ecological safety for non-target organisms,the M2CryAb-vip3A protein was prepared and purified with the purity at 85.0％to treat the adults of the three above-mentioned species in this study.A comprehensive evaluation of the protein's potential effects on their growth,develop-ment,and reproduction was conducted by systematically measuring adult mortality,pre-oviposition period,total egg production,body weight,and body length.The results showed that after M2CryAb-vip3A treatment,the sur-vival rates of P.japonica,C.nipponensis,and F.candida adults were(96.67±0.00)％,(96.67±3.33)％and(91.67±0.00)％,respectively.Furthermore,no significant differences were observed between the treatment groups and the negative control group in terms of pre-oviposition period,total egg production,or body weight.These results demonstrated that M2CryAb-vip3A posed no significant safety risks to P.japonica,C.nipponensis,or F.candida,with no adverse effects on their growth,development,or reproductive capacity.These findings pro-vided critical data support for the ecological safety assessment of the M2CryAb-vip3A insecticidal fusion protein and established a theoretical foundation for optimizing sustainable agricultural pest management strategies.关键词
融合杀虫蛋白M2CryAb-vip3A/非靶标生物/龟纹瓢虫/日本通草蛉/白符跳/生态安全性Key words
Fusion insecticidal protein M2CryAb-vip3A/Non-target organisms/Propylea japonica/Chrysoperla nipponensis/Folsomia candida/Ecological safety分类
植物保护学引用本文复制引用
张华,王文良,赵启辉,丁照华,岳润清..融合杀虫蛋白M2CryAb-vip3A对非靶标生物龟纹瓢虫、日本通草蛉和白符跳的影响[J].山东农业科学,2025,57(10):130-136,7.基金项目
山东省农业科学院农业科技创新工程项目(CXGC2025H06,CXGC2025F02) （CXGC2025H06,CXGC2025F02）
山东省自然科学基金面上项目(ZR2022MC206) （ZR2022MC206）
山东省农业产业技术体系项目(SDAIT-02-04) （SDAIT-02-04）
泰山产业领军人才项目(tscx202408169) （tscx202408169）
