山东农业科学2025，Vol.57Issue(10)：143-148,6.DOI:10.14083/j.issn.1001-4942.2025.10.018
花椒黑霉病病原菌的分离鉴定及室内防治药剂筛选
Isolation and Identification of Pathogenic Bacteria of Zanthoxylum nigrosis and Indoor Screening of Control Agents
摘要
Abstract
To identify the primary pathogenic fungus responsible for Zanthoxylum black mold disease,this study employed tissue isolation,Koch's postulates,morphological and molecular biological methods,and myce-lial growth rate assays to isolate and purify the pathogen,determine its pathogenicity,clarify its taxonomic sta-tus,and screen potential fungicides under laboratory conditions.As a result,a dominant pathogenic strain desig-nated as HJ-6 was isolated from infected leaves and identified as Aspergillus niger.The results of indoor toxicity tests revealed varying inhibitory effects of eight fungicides against A.niger,and the efficacy ranked in descend-ing order of flusilazole＞oxpoconazole＞fluoxastrobin＞prochloraz＞pyraclostrobin＞trifloxystrobin＞pyra-clostrobin＞Pseudomonas fluorescens.Flusilazole exhibited the strongest inhibition(EC50=0.082 7),followed by oxpoconazole(EC50=0.316 9),fluoxastrobin(EC50=0.371 5),and prochloraz(EC50=0.593 1).In contrast,pyraclostrobin,trifloxystrobin and pyraclostrobin showed notably weaker effects and did not recommend for field applications.The four selected fungicides demonstrated high efficacy and low toxicity,providing practical guid-ance for the control of Zanthoxylum black mold disease in actual production.关键词
花椒/黑霉病/科赫氏法则/黑曲霉/毒力测定Key words
Zanthoxylum nigrosis/Black mold disease/Koch's rule/Aspergillus niger/Virulence test分类
植物保护学引用本文复制引用
田凤鸣,陈强,马苗,王国斌,叶文斌..花椒黑霉病病原菌的分离鉴定及室内防治药剂筛选[J].山东农业科学,2025,57(10):143-148,6.基金项目
国家自然科学基金地区基金项目(12061065) （12061065）
陇南市科技计划项目(2021-13,2023-S.QKJ-32) （2021-13,2023-S.QKJ-32）
甘肃省高校教师创新基金项目(2025B-458) （2025B-458）
校级研究机构科研项目(2024B-01) （2024B-01）
陇南师范学院植物学重点学科建设经费项目 （）
