生命科学研究2025，Vol.29Issue(5)：383-388,6.DOI:10.16605/j.cnki.1007-7847.2025.03.0128
不同抗体激活时长对CD8+T细胞表型及慢病毒转导效率的影响
Effects of Antibody Activation Duration on CD8+T Cell Phenotype and Lentivirus Transduction Efficiency
摘要
Abstract
To investigate the effects of CD3/CD28 antibody activation duration on CD8+T cell phenotype and lentiviral transduction efficiency,naïve CD8+T cells were activated with CD3/CD28 antibodies for 24 h,48 h,or 72 h.Phenotypic changes including cell proliferation status and the expression levels of surface molecules CD44,CD62L,and CD69 were detected by flow cytometry.The activated cells were then infected with lentivirus,and transduction efficiency at different activation time points was evaluated using both live-cell imaging and flow cytometry.The results showed that the proliferation rate of CD8+T cells in the 24-hour group was significantly higher than that in the 48-hour and 72-hour groups.For surface molecule ex-pression,the expression level of CD44 in the 24-hour group was significantly higher than that in the non-activated group.When the activation duration extended to 48 h,CD44 expression was further significantly upregulated,but showed no significant difference to that in the 72-hour group.CD62L expression remained at a relatively high level before activation,and was slightly downregulated after 48 h of activation,but re-mained stable among the 0-hour,24-hour,and 72-hour groups.The expression of CD69 in the 24-hour group was significantly higher than that in the non-activated group,but there was no significant difference a-mong the 24-hour,48-hour,and 72-hour groups.For lentiviral transduction efficiency,both the 48-hour and 72-hour groups showed a significant decrease compared with the 24-hour group.This study demon-strates that 24 h is an ideal activation duration,substantially improving CD8+T cell proliferation,activation level,and lentiviral transduction efficiency upon CD3/CD28 antibody stimulation.关键词
CD3/CD28抗体/激活时长/CD8+T细胞/表型/慢病毒转导Key words
CD3/CD28 antibodies/activation duration/CD8+T cell/phenotype/lentivirus transduction分类
生物科学引用本文复制引用
黄梅,杨诗羽,林森,刘永达,袁继行..不同抗体激活时长对CD8+T细胞表型及慢病毒转导效率的影响[J].生命科学研究,2025,29(5):383-388,6.基金项目
国家自然科学基金资助项目(82422059,92374119) （82422059,92374119）
