生命科学研究2025，Vol.29Issue(5)：413-418,6.DOI:10.16605/j.cnki.1007-7847.2024.07.0161
RTyper Y27父系排查试剂盒在两种检测平台上的性能研究
Performance Study of the RTyper Y27 Paternity Detection Kit on Two Detection Platforms
摘要
Abstract
To verify the performance of RTyper Y27 paternity detection reagent on both the traditional poly-merase chain reaction-capillary electrophoresis(PCR-CE)platform and the domestic Quick TargSeqTM 1.0 in-tegrated system for rapid forensic DNA analysis,the RTyper Y27 paternity detection kit was used on both detection platforms,and 649 samples were examined for genetic polymorphism,loci detection rate,concor-dance rate,and simulated case detection.The detection results from 546 samples on PCR-CE platform demonstrated that the gene diversity(GD)values of this RTyper Y27 paternity detection reagent were not less than 0.43,with the highest value(0.88)at DYS449 among the 25 gene loci.Both the detection rate and con-cordance rate of 546 samples were 100.00％.The loci detection rate and the concordance rate of 100 samples on the Quick TargSeqTM 1.0 integrated system platform were 96.37％and 99.64％,respectively.Furthermore,both the loci detection rate and concordance rate of three repeated detections on the simulated case were 100.00％.The results demonstrated that the RTyper Y27 paternity detection kit exhibited high gene polymor-phism with accurate and reliable genotyping,supporting rapid integrated testing on domestic Quick TargSeqTM 1.0 system platform.关键词
法医遗传学/快速DNA分型/Y染色体短串联重复序列(Y-STR)/检测性能Key words
forensic genetics/rapid DNA genotyping/Y-chromosomal short tandem repeat(Y-STR)/perfor-mance detection分类
社会科学引用本文复制引用
陈博旭,王道宇,贾振军,庄斌,赵丽健,姚伊人,韩俊萍..RTyper Y27父系排查试剂盒在两种检测平台上的性能研究[J].生命科学研究,2025,29(5):413-418,6.基金项目
2021年北京市现场物证检验工程技术研究中心开放课题(2021CSEEKFKT06) （2021CSEEKFKT06）
中央级公益性科研院所基本科研业务费专项资金项目(2024JB025) （2024JB025）
