生命科学研究2025，Vol.29Issue(5)：419-424,6.DOI:10.16605/j.cnki.1007-7847.2024.11.0194
肉豆蔻酸促进鲜艳乳菇菌丝生长
Myristic Acid Promotes Lactarius vividus Mycelium Growth
摘要
Abstract
Lactarius vividus is a delicious and precious wild edible mushroom with great economic value.However,as an ectomycorrhizal fungus,it depends on plant symbiosis for its life cycle,resulting in high diffi-culty in artificial cultivation.In the mycorrhizal symbiotic system,mycorrhizal fungi must obtain carbon sources from host plants to fulfill their life cycle.Ectomycorrhiza and arbuscular mycorrhiza(AM)are the main types of mycorrhizae.In arbuscular mycorrhizal symbiosis,fatty acids are the major carbon sources transferred from host plant to the fungal partner.Herein,the modified Melin-Norkrans(MMN)culture medium was supplement-ed with myristic acid(MYR)or/and peptone(PEP)as growth-promoting components for L.vividus mycelium growth.Measurement of colony on different modified culture media showed that adding both MYR and PEP to the MMN medium could significantly improve the efficiency of L.vividus growth.Nile red staining of mycelial fatty acids showed that L.vividus mycelia absorbed fatty acids from the culture medium as carbon sources for growth,which suggested that ectomycorrhizal fungi have a nutrient exchange mode similar to that of arbuscular mycorrhizal fungi.The results provide a theoretical basis for further research on mycorrhizal symbiosis and cultivation of L.vividus.关键词
外生菌根/脂肪酸/鲜艳乳菇/培养基优化Key words
ectomycorrhiza/fatty acid/Lactarius vividus/medium optimization分类
生物科学引用本文复制引用
周雨轩,李菁洋,黄晨容,王鹏..肉豆蔻酸促进鲜艳乳菇菌丝生长[J].生命科学研究,2025,29(5):419-424,6.基金项目
大学生创新创业训练计划项目(S202310542065,S202410542279) （S202310542065,S202410542279）
国家自然科学基金资助项目(32300251) （32300251）
湖南省优秀青年基金项目(2024JJ4029) （2024JJ4029）
