实验科学与技术2025，Vol.23Issue(6)：1-8,8.DOI:10.12179/1672-4550.20250253
基于SDS诱导果蝇肠炎模型的番石榴多糖提取及活性组分评价综合实验设计
A Comprehensive Experimental Design for the Extraction of Guava Polysaccharides and the Evaluation of Active Components Using a Drosophila Enteritis Model Induced by SDS
摘要
Abstract
Drosophila melanogaster is one of the ideal models for evaluating the activity of natural products.In this study,a dextran sulfate sodium(SDS)-induced intestinal inflammation model in Drosophila is established to investigate the ameliorative effects on intestinal inflammation of guava extracts obtained by different methods(traditional water extraction and alcohol precipitation versus a complex enzymatic hydrolysis-assisted deep eutectic solvent method)as well as of different fractions after separation and purification.The results show that the guava polysaccharides extracted by the complex enzymatic hydrolysis-assisted deep eutectic solvent method significantly increases the survival rate of SDS-treated flies and reduces the intestinal barrier permeability.After further separation and purification,the GPB-1 fraction outperformes the GPA fraction in prolonging lifespan,reducing intestinal barrier permeability,restoring gut length and enhancing antioxidant capacity.A comprehensive evaluation system for natural product activity is established using Drosophila as a model,covering the entire workflow from polysaccharide extraction,separation,purification to activity evaluation.It integrates multidisciplinary knowledge from biological science,food nutrition,food chemistry and instrumental analysis.This system not only helps students master natural product extraction techniques,activity evaluation methods and data analysis,but also provides a low-cost and ethically friendly platform for hands-on training,thereby promoting students'knowledge transfer and comprehensive skill improvement.关键词
综合实验设计/黑腹果蝇/番石榴多糖/肠道健康/活性评价Key words
comprehensive experimental design/drosophila melanogaster/guava polysaccharides/gut health/activity evaluation分类
轻工纺织引用本文复制引用
李美英,罗皓琳,王弘,徐振林,孙远明..基于SDS诱导果蝇肠炎模型的番石榴多糖提取及活性组分评价综合实验设计[J].实验科学与技术,2025,23(6):1-8,8.基金项目
国家"十四五"重点研发项目(2023YFF1104203) （2023YFF1104203）
广东省重点研发项目(2022B0202020004) （2022B0202020004）
国家自然科学基金(32472339). （32472339）
