沈阳医学院学报2025，Vol.27Issue(6)：574-582,9.DOI:10.16753/j.cnki.1008-2344.2025.06.003
基于多数据库分析TRIM59基因在肝细胞癌中的表达及甲基化水平的临床意义
Analysis of clinical significance of TRIM59 gene expression and methylation in hepatocellular carcinoma based on multi-database
摘要
Abstract
Objective:To investigate the differential expression and methylation level of tripartite-motif protein 59(TRIM59)in hepatocellular carcinoma(HCC)tissues and its clinical significance.Methods:The expression of TRIM59 in HCC tissue and normal liver tissue was analyzed using the TIMER2.0 and University of ALabama at Birmingham CANcer(UALCAN)databases.The prognostic correlation between TRIM59 and HCC patients was investigated through the TIMER2.0 and Kaplan-Meier Plotter databases.The cBioPortal database was utilized to explore the genetic mutations of TRIM59 in HCC and its co-expressed genes.Gene Ontology(GO)analysis and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway analysis of TRIM59 co-expressed genes were conducted using the DAVID database.The CpG sites of TRIM59 were searched in the University of California Santa Cruz(UCSU)database,and the methylation level of TRIM59 and its correlation with HCC patient prognosis were analyzed in the Shiny Methylation Analysis Resource Tool(SMART)database.Results:TRIM59 was highly expressed in HCC tissue and lowly expressed in normal liver tissue.This differential expression was closely related to the prognosis of HCC patients.Based on GO and KEGG enrichment analyses,TRIM59 co-expressed genes were primarily involved in biological processes such as cell cycle regulation and cytoskeletal organization,as well as signaling pathways including TNF signaling,Hippo signaling,and cytoskeletal regulation.In HCC tissues,the expression of TRIM59 was positively correlated with the infiltration of immune cells such as B lymphocytes,CD8+T lymphocytes,and CD4+T lymphocytes.Additionally,the methylation level of TRIM59 was significantly reduced in HCC tissues,and its expression level was inversely correlated with methylation status.HCC patients with high methylation of TRIM59 had a significantly longer prognosis than those with low methylation.Conclusions:TRIM59 exhibits high expression and low methylation levels in HCC tissues,which are closely associated with the prognosis of HCC patients.Furthermore,differential expression of TRIM59 regulated by methylation plays a crucial role in the development and progression of HCC.关键词
TRIM59/肝细胞癌/甲基化/生物信息学/预后Key words
TRIM59/hepatocellular carcinoma/methylation/bioinformatics/prognosis分类
医药卫生引用本文复制引用
郑顺心,徐颖芳,覃认,谢常斌..基于多数据库分析TRIM59基因在肝细胞癌中的表达及甲基化水平的临床意义[J].沈阳医学院学报,2025,27(6):574-582,9.基金项目
广西壮族自治区卫生与健康委员会自筹经费科研课题(No.Z-B20231556) （No.Z-B20231556）
