沈阳医学院学报2025，Vol.27Issue(6)：583-590,8.DOI:10.16753/j.cnki.1008-2344.2025.06.004
趋化因子CXCL6对前列腺癌生物学行为的影响及相关性研究
Effect and correlation of chemokine CXCL6 on biological behavior of prostate cancer
摘要
Abstract
Objective:To investigate the relationship between CXCL6 expression and prostate cancer,as well as its effects on the malignant progression of PC-3 cells and the underlying mechanisms.Methods:Multiple databases were used to analyze CXCL6 expression in prostate cancer.And survival analysis,GO,and KEGG enrichment analysis were conducted.According to the concentration of CXCL6,PC-3 cells were divided into 0,100 and 200 ng/ml CXCL6 groups.The effects of up-regulated CXCL6 expression on PC-3 cell proliferation,migration,apoptosis,cycle,and related genes were investigated using CCK-8,EdU assay,scratch test,flow cytometry,and prostate cancer-targeted PCR array.Results:Database analysis revealed that CXCL6 was significantly down-regulated in prostate cancer(P＜0.05),and patients with low CXCL6 expression had a worse prognosis(P＜0.05).In prostate cancer,proteins associated with CXCL6 expression included CXCL1 and CXCL2,and related genes included MIR325 and RASSF6.GO analysis indicated that differentially expressed genes(DEGs)of CXCL6 were primarily enriched in processes such as response to chemokines and the tubular endoplasmic reticulum network(P＜0.05).KEGG analysis showed that DEGs were mainly enriched in signaling pathways such as the Hippo signaling pathway(P＜0.05).CCK-8,EdU assay,scratch test,and flow cytometry results demonstrated that compared with the 0 ng/ml CXCL6 group,the 100 and 200 ng/ml CXCL6 groups exhibited significantly reduced proliferation and migration rates(P＜0.05),significantly increased apoptosis rate(P＜0.05).And the proportion of S-phase cells in the 200 ng/ml CXCL6 group was lower compared to the 0 ng/ml CXCL6 group(P＜0.05).PCR array results showed that compared with the 0 ng/ml CXCL6 group,the expression of genes such as CDH1 and HAL was increased in the 200 ng/ml CXCL6 group,while the expression of genes such as CD5L and EDNRB was decreased.Conclusion:CXCL6 plays a significant role in the malignant progression of PC-3 cells,and its mechanism may be associated with the methylation of promoters of genes such as RASSF1,RARB,and CDH1.关键词
生物信息学技术/趋化因子CXCL6/前列腺癌/靶向PCR阵列Key words
bioinformatics technology/chemokine CXCL6/prostate cancer/targeted PCR array分类
医药卫生引用本文复制引用
刘慧丽,杨晓帆,蔡阳艳,张春斌..趋化因子CXCL6对前列腺癌生物学行为的影响及相关性研究[J].沈阳医学院学报,2025,27(6):583-590,8.基金项目
2022年福建省自然基金项目(No.2022J01531) （No.2022J01531）
漳州卫生职业学院院级科研项目(No.ZWYZ202308) （No.ZWYZ202308）
漳州卫生职业学院科技创新团队培育计划(No.KJCX-07) （No.KJCX-07）
