沈阳医学院学报2025，Vol.27Issue(6)：603-607,613,6.DOI:10.16753/j.cnki.1008-2344.2025.06.007
电针联合跑台训练对脑缺血大鼠氧化应激损伤的影响及机制
Effects and mechanisms of electroacupuncture combined with running track exercise on oxidative stress injury in cerebral ischaemic rats
摘要
Abstract
Objective:To investigate the effects of scalp acupuncture of Baihui and Qubin acupoints combined with running track exercise on the neurological function of rats with middle cerebral artery occlusion(MCAO)cerebral ischemia and its regulatory effect on the Nrf2/HO-1 pathway by focusing on oxidative stress injury.Methods:A total of 40 male SD rats of SPF grade were selected and the cerebral ischemia model was established by ligation method.Among them,30 rats with successful modeling were randomly divided into the model group,the scalp-acupuncture group,and the combined group,with 10 rats in each group.Another 10 SPF male SD rats were used as the sham operation group.In the scalp-acupuncture group,electroacupuncture was applied to Baihui and Qubin acupoints.In the combined group,both electroacupuncture and running track exercise were applied.The treatment was carried out 6 times/week for a total of 4 weeks.The neurobehavioural function of rats was evaluated by Bederson Neurological Function Assessment.Pathological changes of rat brain tissue were observed by HE staining.SOD,serum MDA and GSH-px levels were detected by kit.The protein expression levels of Nrf2 and HO-1 in the rat brain tissue were detected by Western blot.Results:Compared with the sham-operated group,Bederson's neurological function score in the model group was significantly higher(P＜0.05),the pathological damage of HE-stained brain tissue was obvious,the level of MDA significantly increased(P＜0.05),the levels of SOD and GSH-px decreased(P＜0.05),and the protein expression levels of Nrf2 and HO-1 were significantly reduced(P＜0.05).Compared with the model group,Bederson neurological function scores were reduced in each treatment group(P＜0.05),the brain histopathological damage was alleviated,the level of MDA was reduced(P＜0.05),the levels of SOD and GSH-px was elevated(P＜0.05),and the protein expression levels of Nrf2 and HO-1 were significantly increased(P＜0.05).The Bederson neurological function score and MDA level in the combined group were lower than those in the scalp-acupuncture group(P＜0.05),and the levels of SOD and GSH-px and the expression levels of Nrf2 and HO-1 proteins were significantly higher than those in the scalp-acupuncture group(P＜0.05).Conclusions:Scalp acupuncture can improve the neurobehavioural dysfunction and pathological damage caused by cerebral ischemia,increase the antioxidant capacity,and the specific mechanism may be related to the activation of the Nrf2/HO-1 pathway.And scalp acupuncture combined with running track exercise has a synergistic effect and is more advantageous for treatment.关键词
脑缺血/电针/运动/氧化应激/Nrf2/HO-1Key words
cerebral ischaemia/electroacupuncture/exercise/oxidative stress/Nrf2/HO-1分类
临床医学引用本文复制引用
赵英子,路岩,王秀珍..电针联合跑台训练对脑缺血大鼠氧化应激损伤的影响及机制[J].沈阳医学院学报,2025,27(6):603-607,613,6.基金项目
通用医疗科研基金项目(No.TYYLKJJ-2022-022) （No.TYYLKJJ-2022-022）
