摘要
Abstract
Objective To explore the mechanism by which the red nocardia cell wall skeleton(Nr-CWS)promotes healing of scald wounds.Methods A model of deep second-degree burn in mice was established.A total of 30 male BALB/C mice were randomly divided into the Nr-CWS group and the normal saline group.Dissolve 60 μg Nr-CWS in 1 mL of physiological saline,and applied it externally to the mouse wounds.Keep the exposed wounds dry.The control group was treated by applying 1mL of physiological saline to the mouse wounds,while keeping the exposed wounds dry.Both groups of mice had the medication changed once a day,and the treatment was continued for 22 days.The healing status of the wounds was recorded using a camera,and skin tissue samples from the wounds were collected on days 3,7,14,and 21 for making frozen sections.Pathological analysis was conducted using Hematoxylin and Eosin staining(HE staining)and Masson stai-ning.Immunohistochemical(IHC)staining was conducted to observe changes in CCR7,CD206,LY6G,and CD31 expression at the wound site.Enzyme-Linked Immunosorbent Assay(ELISA)was used to detect the levels of TNF-α and TGF-β in the wound.Results After treatment,the Nr-CWS group exhibited earlier burn wound healing time and higher healing rates compared to the control group.On Day 3,the positive rate of LY6G+neutrophils in the Nr-CWS group was significantly lower than that in the control group[(16.00±2.94)%vs.(31.00±2.93)%,P<0.01].Furthermore,the peaks of M1 and M2 macrophages occurred earlier in the Nr-CWS group compared to the control group(P<0.01).During the healing process of the scald wounds,the expression level of TNF-α and the number of M1 type macrophages both showed a downward trend,while the positive rate of CD31 cells,the expression level of TGF-β,and the number of M2 type macro-phages all showed an initial increase followed by a decrease.Conclusion The Nr-CWS can accelerate scald wound healing,reduce the aggregation of neutrophils in the wound,and promote macrophages proliferation of wound in the early stages of scald wound,facilitating the transformation of M1 type macrophages to M2 type macrophages,and promoting the transition of the wound from the inflammatory reaction stage to the repair stage.关键词
红色诺卡菌细胞壁骨架/烫伤创面/创面愈合/巨噬细胞/极化/炎症Key words
Red nocardia cell wall skeleton/Scald wound/Wound healing/Macrophage/Polari-zation/Inflammation分类
临床医学
