湖北科技学院学报（医学版）2025，Vol.39Issue(6)：461-465,5.DOI:10.16751/j.cnki.2095-4646.2025032803
多穗石柯总黄酮提取物对H2O2诱导人脐静脉内皮细胞铁死亡的改善作用
The Ameliorative Effect of Total Flavonoid Extracts of Lithocarpus Polystachyus Rehd on Ferroptosis Induced by H2O2 in Human Umbilical Vein Endothelial Cells
摘要
Abstract
Objective To explore the ameliorative effect of total flavonoid extract of Lithocarpus polystachyus Rehd(TC)on H2O2-induced ferroptosis in human umbilical vein endothelial cells(HUVECs).Methods Blood was taken from the abdominal aorta of rats after administration of TC to prepare drug-containing rat serum,and the effective concentration for cultured cells was detemined by CCK-8 method and its protective effect on H2O2-induced injury in HUVECs were evaluated.The protein expression level of Acyl-CoA synthetase long-chain 4(ACSL4)and Cystathionine β-synthase(CBSL)in cells of each group were detected by Western blot.The RNA of the cells was extracted to carry the transcriptome sequencing and bioinformatics analysis.Results Cell viability values were significantly higher in the 10％drug-containing rat serum group.CBSL protein was down-regulated in the presence of H2O2-induced damage and cell viability values were signifi-cantly higher after treatment with TC or Fer-1.Transcriptome sequencing results showed that CBSL was significantly down-regulated and closely related to sulfur compound metabolism.Additionally,Western blot results showed that TC and Fer-1 shared the similar effects to de-crease the expression level of CBSL proteins and increase the expression level of ACSL4 proteins compared with H2O2 group.Conclusion TC attenuates oxidative stress-mediated ferroptosis in endothelial cells and shows the potential protective effects on blood vessels.关键词
多穗石柯/内皮细胞/氧化应激/铁死亡Key words
Lithocarpus polystachyus Rehd/Endothelial cells/Oxidative stress/Ferroptosis分类
医药卫生引用本文复制引用
孙艺瑄,雷敏,季慧敏,蒋越,李奥迪,陈清杰..多穗石柯总黄酮提取物对H2O2诱导人脐静脉内皮细胞铁死亡的改善作用[J].湖北科技学院学报（医学版）,2025,39(6):461-465,5.基金项目
国家自然科学基金一般面上项目(82270892) （82270892）
