湖北科技学院学报（医学版）2025，Vol.39Issue(6)：466-470,5.DOI:10.16751/j.cnki.2095-4646.2025030707
青砖茶提取物对溃疡性结肠炎小鼠治疗作用研究
The Therapeutic Effect of Qing Dark Tea Extract on Ulcerative Colitis in Mice
摘要
Abstract
Objective To investigate the therapeutic effects and potential mechanism of Qingzhuan Dark tea extract(QDTE)on ul-cerative colitis(UC)in mice induced by dextran sulfate sodium(DSS).Methods A UC model was established in C57BL/6 mice by 3％DSS,and the mice were intervened with 200mg/(kg·d)and 400mg/(kg·d)of QDTE,respectively.The therapeutic effect of QDTE was evaluated by monitoring body weight changes,disease activity index(DAI),and colon length.The levels of inflammatory factors(IL-6,TNF-α)in serum and colon tissue were detected by enzyme-linked immunosorbent assay(ELISA),and the expression changes of zonula occlu-dens-1(ZO-1)and occludin were analyzed by immunohistochemistry.Results Compared with the Model group,QDTE intervention signifi-cantly alleviated the weight loss of UC mice(P＜0.05),reduced DAI scores(P＜0.05),and increased colon length(P＜0.05).In addi-tion,QDTE significantly inhibited the release of pro-inflammatory factors IL-6 and TNF-α in the colon and serum(P＜0.05),and upregu-lated the expression of ZO-1 and Occludin in the intestinal mucosa(P＜0.05).Conclusion QDTE effectively alleviates the symptoms of DSS-induced UC in mice by inhibiting inflammatory responses and enhancing intestinal barrier function,indicating its potential application value as a functional food or adjuvant therapy for UC.关键词
青砖茶提取物/葡聚糖硫酸钠/溃疡性结肠炎/炎症Key words
Qingzhuan dark tea extract/Dextran sulfate sodium/Ulcerative colitis/Inflammation引用本文复制引用
赵依叶,叶丹,权浩洋,苗潇磊,郑敏..青砖茶提取物对溃疡性结肠炎小鼠治疗作用研究[J].湖北科技学院学报（医学版）,2025,39(6):466-470,5.基金项目
国家自然科学基金重大科研仪器研制项目(81727805) （81727805）
咸宁市科技研发重点专项项目(2021SFYF003) （2021SFYF003）
湖北科技学院科研创新团队项目(2023T10) （2023T10）
