湖北科技学院学报（医学版）2025，Vol.39Issue(6)：484-488,5.DOI:10.16751/j.cnki.2095-4646.2025042406
二甲双胍通过调控Nur77抑制巨噬细胞极化和炎症反应的研究
Metformin Inhibited Macrophage Polarization and Inflammatory Response by Regulating Nur77
摘要
Abstract
Objective To investigate the role and mechanism of metformin(Met)in regulating macrophage polarization and inflam-matory responses.Methods Using lipopolysaccharide(LPS)-stimulated RAW 264.7 macrophages as a model,cells were divided into six groups:normal control group(Con),the LPS group,the LPS+metformin group(2mmol/L),the LPS+metformin group(10mmol/L),the LPS+metformin group(50mmol/L),and the LPS+metformin group(100mmol/L).Cell morphology was observed under a microscope and cell viability was determined by CCK8.The secretion of IL-1β,IL-6 and TNF-α were detected using ELISA.The protein expression levels of Nur77 and NF-κB were detected by Western blot.Results The microscopic results showed that compared with the normal control group,LPS stimulation activated macrophages and promoted polarization toward the M1 phenotype.Metformin intervention ameliorated LPS-induced mac-rophage damage and restored normal cell morphology.The CCK8 results showed that treatment with 50mmol/L metformin for 12 hours yiel-ded the highest cell viability.ELISA results showed that compared with the normal control group,LPS significantly increased the levels of IL-1β,IL-6,and TNF-α(all P＜0.0001),which were most markedly reduced by the addition of 50mmol/L metformin(P＜0.0001).Western blot results showed that compared with the normal control group,LPS significantly upregulated NF-κB protein expression(P＜0.0001)and downregulated Nur77 expression(P＜0.05).In contrast,compared with the LPS group,metformin treatment significantly decreased NF-κB expression(P＜0.001)and increased Nur77 expression(P＜0.001).Conclusion Metformin can enhance the anti-inflammatory ability of macrophages and affect macrophage polarization by regulating Nur77,thereby inhibiting inflammatory responses.These findings provide new ideas for targeted treatment of various inflammatory diseases.关键词
二甲双胍/Nur77/巨噬细胞/极化/炎症Key words
Metformin/Nur77/Macrophage/Polarization/Inflammation分类
医药卫生引用本文复制引用
潘欣怡,周震,付晓俐,吴婉秋,包永芬,瞿利花,单士刚,黄丽琼..二甲双胍通过调控Nur77抑制巨噬细胞极化和炎症反应的研究[J].湖北科技学院学报（医学版）,2025,39(6):484-488,5.基金项目
湖北省科技创新示范项目(2022BCE052) （2022BCE052）
