湖北科技学院学报（医学版）2025，Vol.39Issue(6)：489-493,5.DOI:10.16751/j.cnki.2095-4646.2025041515
芹菜素抑制AngⅡ诱导心肌成纤维细胞胶原蛋白合成机制研究
Apigenin Inhibits Ang Ⅱ induced Collagen Synthesis in Cardiac Fibroblasts by Regulating QKI Expression
摘要
Abstract
Objective To investigate the effects of apigenin(APi)regulating of tremor gene quaking(QKI)expression on angioten-sin Ⅱ(Ang Ⅱ)induced collagen synthesis in mouse myocardial fibroblasts(MCFs).Methods MCFs were pretreated by APi for 1h or in-fected with adenovirus Ad_QKI for 12h,followed by Ang Ⅱ supplementation and culture for another 24 h.Western blot was used to detect the expression of Col Ⅰ/Ⅲ proteins and QKI proteins in MCFs.Cell viability was detected by CCK-8 kit.Results 1μmol/L Ang Ⅱ signifi-cantly increased the synthesis of Col Ⅰ(P＜0.01)and Col Ⅲ(P＜0.001)proteins and significantly decreased the expression of QKI pro-teins in MCFs(P＜0.05).Adenovirus Ad_QKI infection of MCFs upregulated QKI protein expression and significantly inhibited Ang Ⅱ in-duced Col Ⅰ and Col Ⅲ protein expression.APi treatment at a concentration of 10μmol/L or overexpression of QKI could significantly in-hibit the increase in the viability of MCFs cells induced by Ang Ⅱ(1μmol/L).Furthermore,APi not only inhibited the expression of Col Ⅰand Col Ⅲ proteins induced by Ang Ⅱ(1μmol/L)in MCFs,but also attenuated the down-regulation of QKI protein expression induced by Ang Ⅱ(1 μmol/L).Conclusion APi promotes QKI expression and alleviates the increase induced by Ang Ⅱ in the viability of MCFs and the synthesis of Col Ⅰ and Col Ⅲ proteins.关键词
芹菜素/QKI/血管紧张素Ⅱ/胶原蛋白合成/心肌成纤维细胞Key words
Apigenin/Quaking/Angiotensin Ⅱ/Collagen synthesis/Cardiac fibroblast分类
医药卫生引用本文复制引用
贾宏玉,宋晨晨,杨君娴,阚洪爽,王平芳,杨晓松,陈红光..芹菜素抑制AngⅡ诱导心肌成纤维细胞胶原蛋白合成机制研究[J].湖北科技学院学报（医学版）,2025,39(6):489-493,5.基金项目
国家自然科学基金面上项目(82073852) （82073852）
咸宁市自然科学基金(2023ZRKX086) （2023ZRKX086）
湖北科技学院糖尿病心脑血管病变湖北省重点实验室糖尿病专项(2024TNB01) （2024TNB01）
教育部产学合作协同育人项目(231107604083103) （231107604083103）
